|
|
Materials
You will need:
- Deionized water
- Tryptone
- NaCl
- Yeast Extract
- Agar
Procedure
- Combine 10g tryptone, 5g yeast extract, 10g NaCl, 15g agar, and 950 mL of deionized water into a large beaker/Erlenmeyer flask.
- Mix until dissolved using a stir bar.
- Check to see if the pH is 7.0 using a pH meter. If needed, add 5N NaOH to adjust the pH to 7.0.
- Fill solution to 1L with deionized water.
- Autoclave.
- Let it cool, but not cool enough to let it solidify. It is recommended to keep it in 45 degree waterbath.
- Add the appropriate amount of antibiotic of your choice. Recommended amounts are posted on openwetware.org. Mix antibiotic.
- Pour into sterilized plates.
Purpose
Nutrient agar plates are used to grow and select for desired microbes.
References
- Written by David Larsen 9/11/08
- Cold Spring Harbor protocol
|
|
|
|
|
|
|
 |
|---|
|
We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!
We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)
|
|
|
"
