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Team:LMU-Munich/Notebook/Protocols/17 Special thermal cycler programs/Touch62
From 2010.igem.org
(Difference between revisions)
(New page: {{:Team:LMU-Munich/Templates/Page Header}} <b>PCR program: Touch-Down</b> ::{| |- |95°C |1 min |- |95°C |30 sec |- |62°C |30 sec |- |72°C |30 sec |- |return to step 2 for 2 cycles |-...) |
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|30 sec | |30 sec | ||
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|72°C | |72°C | ||
|10 min | |10 min | ||
Revision as of 14:03, 2 September 2010
PCR program: Touch-Down
95°C
1 min
95°C
30 sec
62°C
30 sec
72°C
30 sec
return to step 2 for 2 cycles
95°C
30 sec
60°C
30 sec
72°C
30 sec
return to step 6 for 2 cycles
95°C
30 sec
58°C
30 sec
72°C
30 sec
return to step 10 for 2 cycles
95°C
30 sec
56°C
30 sec
72°C
30 sec
return to step 14 for 2 cycles
95°C
30 sec
54°C
30 sec
72°C
30 sec
return to step 18 for 2 cycles
95°C
30 sec
52°C
30 sec
72°C
30 sec
return to step 22 for 2 cycles
95°C
30 sec
50°C
30 sec
72°C
30 sec
return to step 26 for 2 cycles
95°C
30 sec
55°C
30 sec
72°C
30 sec
72°C
10 min
12°C
forever
