Team:TU Delft/1 September 2010 content
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Revision as of 10:46, 2 September 2010
Science Center Opening
Today was the official opening of the Science Center - TU Delft's technology museum. It was very exciting, because it was the first time the whole team saw the BioTech room we helped develop. It contains a lab-table, a wall displaying images of microorganisms, and a flow cabinet in which short movies are displayed on various subjects ranging from extremofiles to GFP and penicillin. Eva and Nadine star in these movies! Further more we also interviewed Karel Luyben, Rector Magnificus of TU Delft.
Lab work
Alkane degradation
Today another DLT - we will stick a strong promoter (J13002) in front of ladA
# | Sample | Enzyme 1 | Enzyme 2 | Enzyme 3 | Buffer | BSA | Needed fragment |
1 | LadA | XbaI | PstI | AseI | 3 (Biolabs) | ✓ | ‘X – ladA – P’ |
2 | J13002 | SpeI | PstI | ✗ | 2 (Biolabs) | ✓ | ‘P – pSB1A2 – J13002 – S’ |
Directly following the digestion, the products were ligated for 1 hour:
# | BioBrick | Fragment 1 | Fragment 2 |
1 | pSB1A2 - J13002 - ladA | ‘X – ladA – P’ | ‘P – pSB1A2 – J13002 – S’ |
Lane description
# | Description | Expected Length (bp) | Status |
0 | Smartladder (5μl) | ||
1 | 305 | 5613 | ✓ |
2 | 329 | 3588,2105 | ✗ |
3 | I13401 | 2936 | ✗ |
4 | 331 | 2105, 911 | ✗ |
5 | 331 | 2105, 911 | ✗ |
6 | Smartladder (5μl) | ||
7 | undigested 025 (#2) | ✓ | |
8 | 025 (#2) | 2293, 1262 | ✗ |
9 | undigested 025 (#8) | ✓ | |
10 | 025 (#8) | 2293, 1262 | ✗ |
11 | Smartladder (5μl) | ||
12 | ‘X – ladA – P’ | ✓ | |
13 | ‘P – pSB1A2 – J13002 – S’ | ✓ (low concentration) |