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From 2010.igem.org

Science Center Opening

Today was the official opening of the Science Center - TU Delft's technology museum. It was very exciting, because it was the first time the whole team saw the BioTech room we helped develop. It contains a lab-table, a wall displaying images of microorganisms, and a flow cabinet in which short movies are displayed on various subjects ranging from extremofiles to GFP and penicillin. Eva and Nadine star in these movies! Further more we also interviewed Karel Luyben, Rector Magnificus of TU Delft.

View more photos on our gallery

Lab work

Alkane degradation

Today another DLT - we will stick a strong promoter (J13002) in front of ladA

#	Sample	Enzyme 1	Enzyme 2	Enzyme 3	Buffer	BSA	Needed fragment
1	LadA	XbaI	PstI	AseI	3 (Biolabs)	✓	‘X – ladA – P’
2	J13002	SpeI	PstI	✗	2 (Biolabs)	✓	‘P – pSB1A2 – J13002 – S’

Directly following the digestion, the products were ligated for 1 hour:

#	BioBrick	Fragment 1	Fragment 2
1	pSB1A2 - J13002 - ladA	‘X – ladA – P’	‘P – pSB1A2 – J13002 – S’

Digestion check:

Lane description

#	Description	Expected Length (bp)	Status
0	Smartladder (5μl)
1	305	5613	✓
2	329	3588,2105	✗
3	I13401	2936	✗
4	331	2105, 911	✗
5	331	2105, 911	✗
6	Smartladder (5μl)
7	undigested 025 (#2)		✓
8	025 (#2)	2293, 1262	✗
9	undigested 025 (#8)		✓
10	025 (#8)	2293, 1262	✗
11	Smartladder (5μl)
12	‘X – ladA – P’		✓
13	‘P – pSB1A2 – J13002 – S’		✓ (low concentration)