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UTDallas/16 August 2010
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*We digested some of the parts and then gel purified them. All but two worked. | *We digested some of the parts and then gel purified them. All but two worked. | ||
*We incubated some of the glycerol stock of the two that did not work. | *We incubated some of the glycerol stock of the two that did not work. | ||
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| + | Image 1: There is a 2-log DNA ladder, then there is not a correct band for Pu, then there is a band for PyeaR | ||
| + | [[Image:8-16.JPG |500px | center ]] | ||
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| + | Image 2: There is a 2-log DNA ladder, then Pr+XylR correct, then Terminator incorrect, then Red correct, then GFP correct, then Orange correct, then mRFP1 correct, and then a 1 kb DNA ladder | ||
| + | [[Image:8-16 (2).JPG |500px | center ]] | ||
Latest revision as of 01:33, 2 September 2010
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August 16, 2010
- We started the cloning procedure.
- We digested some of the parts and then gel purified them. All but two worked.
- We incubated some of the glycerol stock of the two that did not work.
Image 1: There is a 2-log DNA ladder, then there is not a correct band for Pu, then there is a band for PyeaR
Image 2: There is a 2-log DNA ladder, then Pr+XylR correct, then Terminator incorrect, then Red correct, then GFP correct, then Orange correct, then mRFP1 correct, and then a 1 kb DNA ladder
.JPG)
