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Team:Newcastle/27 August 2010
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==Results== | ==Results== | ||
| - | [[Image:Newcastle_controlgel_270810.jpg|500px]] | + | |
| - | + | [[Image:Newcastle_controlgel_270810.jpg|500px|centre]] | |
| + | '''Figure 1''': Gel electrophoresis of the amplified PCR products and restriction digest | ||
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| + | *'''Lane 1''': 1 Kb ladder | ||
| + | *'''Lane 2''': pSB1C3 (for Subtilin Immunity) | ||
==Discussion== | ==Discussion== | ||
Revision as of 10:37, 27 August 2010
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Contents |
PCR and digestion controls
Aims
Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that there is nothing wrong with any of our enzymes or ...
Materials and Protocols
Please refer to the restriction digest, Phusion PCR and gel electrophoresis protocols.
...
Results
Figure 1: Gel electrophoresis of the amplified PCR products and restriction digest
- Lane 1: 1 Kb ladder
- Lane 2: pSB1C3 (for Subtilin Immunity)
Discussion
Worked correctly.. nothing is wrong with our enzymes.. in the case of our rocF plasmid PCRs, it must be the primers which are the problem.. new ones are supposed to be arriving today.. in the other cases, repeat..
yneA
   
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