"
Team:Newcastle/27 August 2010
From 2010.igem.org
(Difference between revisions)
Swoodhouse (Talk | contribs) (New page: {{Team:Newcastle/mainbanner}} =PCR and digestion controls= ==Aims== Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that ...) |
Swoodhouse (Talk | contribs) (→Results) |
||
| Line 10: | Line 10: | ||
==Results== | ==Results== | ||
[[Image:Newcastle_controlgel_270810.jpg|500px]] | [[Image:Newcastle_controlgel_270810.jpg|500px]] | ||
| + | ... | ||
| + | |||
| + | ==Discussion== | ||
| + | Worked correctly.. nothing is wrong with our enzymes.. in the case of our ''rocF'' plasmid PCRs, it must be the primers which are the problem.. new ones are supposed to be arriving today.. in the other cases, repeat.. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 10:14, 27 August 2010
| |||||||||||||
| |||||||||||||
Contents |
PCR and digestion controls
Aims
Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that there is nothing wrong with any of our enzymes or ...
...
Results
...
Discussion
Worked correctly.. nothing is wrong with our enzymes.. in the case of our rocF plasmid PCRs, it must be the primers which are the problem.. new ones are supposed to be arriving today.. in the other cases, repeat..
   
|
    
|
