Team:TU Delft/18 June 2010 content

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We had a nice slow day, finishing some small experiments and reorganizing our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
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=Cleaning=
 +
After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
 +
 
 +
We also booked a hotel in Boston!
 +
 
 +
=Lab work=
 +
 
 +
==Emulsifier==
 +
 
 +
Pieter worked on the emulsifying activity assay. Four samples were tested:
 +
 
 +
1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.
 +
 
 +
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
 +
|'''#'''
 +
|'''Amount of SDS (%)'''
 +
|'''Hexalkane (mL)'''
 +
|-
 +
|1
 +
|0
 +
|0
 +
|-
 +
|2
 +
|0
 +
|0.02
 +
|-
 +
|3
 +
|0.01
 +
|0.02
 +
|-
 +
|4
 +
|1
 +
|0.02
 +
|}
 +
 
 +
2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.
 +
 
 +
3. Shake 1 hour with 200 strokes/min at 30°C
 +
 
 +
4. Measure absorbance OD600
 +
 
 +
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
 +
|'''#'''
 +
|'''A600'''
 +
|-
 +
|1
 +
|0
 +
|-
 +
|2
 +
|0
 +
|-
 +
|3
 +
|0.4
 +
|-
 +
|4
 +
|0.2
 +
|}
 +
 
 +
==Biobricks==
 +
By Hugo
 +
 
 +
I've got some colonies in the plates, however there's contamination with some yellow colonies :s
 +
Probably, we will have to prepare a new stock of competent cells.

Latest revision as of 08:28, 26 August 2010

Contents

Cleaning

After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.

We also booked a hotel in Boston!

Lab work

Emulsifier

Pieter worked on the emulsifying activity assay. Four samples were tested:

1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.

# Amount of SDS (%) Hexalkane (mL)
1 0 0
2 0 0.02
3 0.01 0.02
4 1 0.02

2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.

3. Shake 1 hour with 200 strokes/min at 30°C

4. Measure absorbance OD600

# A600
1 0
2 0
3 0.4
4 0.2

Biobricks

By Hugo

I've got some colonies in the plates, however there's contamination with some yellow colonies :s Probably, we will have to prepare a new stock of competent cells.

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