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Team:TU Delft/11 June 2010 content
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(New page: ==Labwork== Today we purified a mass stock of all the plasmids we will be using for BioBrick assembly. Next to that we also started digesting the first parts we will be characterizing. ==...) |
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| - | == | + | =Lab work= |
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| - | ==Spring Workshop= | + | ==BioBrick stocks== |
| - | Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the | + | Today we performed a [[Team:TU_Delft/protocols/mini-prep_plasmid_isolation|Qiagen Mini-prep plasmid isolation]] of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=8_June_2010 vectors] we grew overnight. We saved 3 mL of the bacterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. |
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| + | The following plasmid concentrations were obtained: | ||
| + | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
| + | |'''BioBrick''' | ||
| + | |'''Concentration (ng/μL)''' | ||
| + | |- | ||
| + | |pSB3T5 | ||
| + | |266.0 | ||
| + | |- | ||
| + | |pSB1T3 | ||
| + | |414.0 | ||
| + | |- | ||
| + | |pSB1C3 | ||
| + | |284.7 | ||
| + | |- | ||
| + | |pSB3C5 | ||
| + | |329.3 | ||
| + | |- | ||
| + | |pSB1K3 | ||
| + | |270.1 | ||
| + | |- | ||
| + | |pSB1A3 | ||
| + | |271.9 | ||
| + | |- | ||
| + | |I13401 | ||
| + | |232.1 | ||
| + | |} | ||
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| + | [[Image:P1010002_small.jpg|200px|thumb|left|Ramon & Hugo in the lab]] | ||
| + | [[Image:P1010005_small.jpg|180px|thumb|left|Thias doing labwork]] | ||
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| + | ==Characterization of Anderson RBS sequences== | ||
| + | Next to that we also started digesting the first parts we will be characterizing. | ||
| + | |||
| + | ==Strain Characterization== | ||
| + | By Hugo | ||
| + | |||
| + | I prepared the cell bank according to the following procedure: | ||
| + | |||
| + | * Spin 5 ml of the overnight culture | ||
| + | * Add 500 ul of LB medium | ||
| + | * Add 500 ul of 80% glycerol | ||
| + | * Store the tube at -80ºC | ||
| + | |||
| + | Now, we have a cell bank ready to be used!!! | ||
| + | |||
| + | =Spring Workshop= | ||
| + | |||
| + | Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep. | ||
| + | |||
| + | [[Image:IGEM_photo1.jpg|300px|thumb|left|Leaving Delft]] | ||
| + | [[Image:DSCF2276_small.jpg|300px|thumb|left|Dinner on the way to Évry, while watching the soccer match in Gent]] | ||
| + | [[Image:DSCF2277_small.jpg|300px|thumb|left|pizza tast good]] | ||
| + | [[Image:DSC4777_small.jpg|300px|thumb|left|Dinner on the way to Évry]] | ||
| + | [[Image:DSC4779_small.jpg|300px|thumb|left|Last car arriving at the hotel in Évry]] | ||
Latest revision as of 16:09, 25 August 2010
Contents |
Lab work
BioBrick stocks
Today we performed a Qiagen Mini-prep plasmid isolation of the vectors we grew overnight. We saved 3 mL of the bacterial cells to make -80 °C stocks.
The following plasmid concentrations were obtained:
| BioBrick | Concentration (ng/μL) |
| pSB3T5 | 266.0 |
| pSB1T3 | 414.0 |
| pSB1C3 | 284.7 |
| pSB3C5 | 329.3 |
| pSB1K3 | 270.1 |
| pSB1A3 | 271.9 |
| I13401 | 232.1 |
Characterization of Anderson RBS sequences
Next to that we also started digesting the first parts we will be characterizing.
Strain Characterization
By Hugo
I prepared the cell bank according to the following procedure:
- Spin 5 ml of the overnight culture
- Add 500 ul of LB medium
- Add 500 ul of 80% glycerol
- Store the tube at -80ºC
Now, we have a cell bank ready to be used!!!
Spring Workshop
Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep.
