From 2010.igem.org

Lab work

BioBrick stocks

Today we performed a Qiagen Mini-prep plasmid isolation of the vectors we grew overnight. We saved 3 mL of the bacterial cells to make -80 °C stocks.

The following plasmid concentrations were obtained:

Ramon & Hugo in the lab

Thias doing labwork

Characterization of Anderson RBS sequences

Next to that we also started digesting the first parts we will be characterizing.

Strain Characterization

By Hugo

I prepared the cell bank according to the following procedure:

• Spin 5 ml of the overnight culture
• Add 500 ul of LB medium
• Add 500 ul of 80% glycerol
• Store the tube at -80ºC

Now, we have a cell bank ready to be used!!!

Spring Workshop

Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep.

Leaving Delft

Dinner on the way to Évry, while watching the soccer match in Gent

pizza tast good

Dinner on the way to Évry

Last car arriving at the hotel in Évry