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Team:Newcastle/23 August 2010
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[[Image:Newcastle Gel 1 - 23-08-2010.jpg|700px|centre]] | [[Image:Newcastle Gel 1 - 23-08-2010.jpg|700px|centre]] | ||
| - | '''Figure 1''': Gel electrophoresis of plasmid pSB1C3 | + | '''Figure 1''': Gel electrophoresis of the amplified linearized plasmid pSB1C3 fragments ran at 4 different melting temperatures, Tms, (50, 60, 65, 70°C). A 1 kb DNA ladder was used on either side of lanes. |
* '''Lane 1''': pSB1C3 fragment amplified at 55°C | * '''Lane 1''': pSB1C3 fragment amplified at 55°C | ||
* '''Lane 2''': pSB1C3 fragment amplified at 55°C | * '''Lane 2''': pSB1C3 fragment amplified at 55°C | ||
Revision as of 10:51, 24 August 2010
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Contents |
pSB1C3 plasmid gel electrophoresis
Aims
The aim of this experiment is to run gel electrophoresis for the extracted and linearized plasmid pSB1C3 fragment which were amplified at 4 different melting temperatures by 4 separate PCR reactions.
Materials and Protocol
Please refer to: Gel electrophoresis for gel electrophoresis protocol.
Result
Figure 1: Gel electrophoresis of the amplified linearized plasmid pSB1C3 fragments ran at 4 different melting temperatures, Tms, (50, 60, 65, 70°C). A 1 kb DNA ladder was used on either side of lanes.
- Lane 1: pSB1C3 fragment amplified at 55°C
- Lane 2: pSB1C3 fragment amplified at 55°C
- Lane 3: pSB1C3 fragment amplified at 60°C
- Lane 4: pSB1C3 fragment amplified at 60°C
- Lane 5: pSB1C3 fragment amplified at 65°C
- Lane 6: pSB1C3 fragment amplified at 65°C
- Lane 7: pSB1C3 fragment amplified at 70°C
- Lane 8: pSB1C3 fragment amplified at 70°C
Discussion
Conclusion
   
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