"
Team:Edinburgh/Modelling/Bacterial
From 2010.igem.org
| Line 123: | Line 123: | ||
<br> | <br> | ||
| - | <p>The second Kappa model created for the project attempted to realise the original vision we held for the system: a composite device based on the tried and tested Elowitz repressilator, combined with three different light-producing and light-sensing systems.</p><br> | + | <p>The second Kappa model created for the project attempted to realise the original vision we held for the system: a composite device based on the tried and tested Elowitz repressilator, combined with three different light-producing and light-sensing systems. The primary objective of the modelling would then be to confirm that the three systems interact with one another in roughly the manner we expect, without undue interference or trouble. We would also try to use the model to analyse the structure of the system and possibly to compare different proposed subsystems against one another, to see which one works better.</p> |
| + | |||
| + | <p>The following sections describe, in turn: the <a href="#Repressilator">repressilator</a> model that forms the core of the system, the <a href="#RedLight">red light</a> production and signal transduction pathways, the <a href="#BlueLight">blue light</a> production and signal transduction pathways, the <a href="#GreenLight">green light</a> production and signal transduction pathways, the <a href="#Results">results</a> obtained by running the simulation, and finally the <a href="#Analysis">analysis</a> of the results obtained.</p><br><br> | ||
<br> | <br> | ||
| Line 171: | Line 173: | ||
<a name="BlueLight" id="BlueLight"></a><h2>The Blue Light Pathway</h2> | <a name="BlueLight" id="BlueLight"></a><h2>The Blue Light Pathway</h2> | ||
| + | <br> | ||
| + | |||
| + | <p>***</p><br> | ||
| + | <br> | ||
| + | |||
| + | <!-- <center><br><br><p><img src="https://static.igem.org/mediawiki/2010/1/19/Ed10-DNA.JPG" width="400px"></p><br> | ||
| + | <p><b>Figure 1:</b> A composite agent with four DNA agents joined together at their upstream and downstream sites, representing the <i>cat-sacB</i> construct and surrounding DNA.</p><br><br></center> | ||
| + | |||
| + | Assumptions. | ||
| + | |||
| + | Describe the components of the pathway. | ||
| + | |||
| + | Results thereof. | ||
| + | |||
| + | --> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <div id="body" style="padding: 0px 60px 10px 60px; height: 1356px"> | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | <a name="GreenLight" id="GreenLight"></a><h2>The Green Light Pathway</h2> | ||
<br> | <br> | ||
Revision as of 09:05, 24 August 2010
Overview: Modelling bacterial BRIDGEs
The second Kappa model created for the project attempted to realise the original vision we held for the system: a composite device based on the tried and tested Elowitz repressilator, combined with three different light-producing and light-sensing systems. The primary objective of the modelling would then be to confirm that the three systems interact with one another in roughly the manner we expect, without undue interference or trouble. We would also try to use the model to analyse the structure of the system and possibly to compare different proposed subsystems against one another, to see which one works better.
The following sections describe, in turn: the repressilator model that forms the core of the system, the red light production and signal transduction pathways, the blue light production and signal transduction pathways, the green light production and signal transduction pathways, the results obtained by running the simulation, and finally the analysis of the results obtained.
The Repressilator
***
