Notebook
| June
|
| M | T | W | T | F | S | S
|
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/1_June_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/2_June_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/3_June_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/4_June_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/5_June_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/6_June_2010&action=edit 6]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/7_June_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/8_June_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/9_June_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/10_June_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/11_June_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/12_June_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/13_June_2010&action=edit 13]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/14_June_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/15_June_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/16_June_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/17_June_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/18_June_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/19_June_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/20_June_2010&action=edit 20]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/21_June_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/22_June_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/23_June_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/24_June_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/25_June_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/26_June_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/27_June_2010&action=edit 27]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/28_June_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/29_June_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/30_June_2010&action=edit 30]
|
|
| July
|
| M | T | W | T | F | S | S
|
|
|
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/1_July_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/2_July_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/3_July_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/4_July_2010&action=edit 4]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/5_July_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/6_July_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/7_July_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/8_July_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/9_July_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/10_July_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/11_July_2010&action=edit 11]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/12_July_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/13_July_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/14_July_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/15_July_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/16_July_2010&action=edit 16]
| [http://2010.igem.org/UTDallas/17_July_2010 17]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/18_July_2010&action=edit 18]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/19_July_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/20_July_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/21_July_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/22_July_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/23_July_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/24_July_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/25_July_2010&action=edit 25]
|
| [http://2010.igem.org/UTDallas/26_July_2010 26]
| [http://2010.igem.org/UTDallas/27_July_2010 27]
| [http://2010.igem.org/UTDallas/28_July_2010 28]
| [http://2010.igem.org/UTDallas/29_July_2010 29]
| [http://2010.igem.org/UTDallas/30_July_2010 30]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/31_July_2010&action=edit 31]
|
|
| August
|
| M | T | W | T | F | S | S
|
|
|
|
|
|
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/1_August_2010&action=edit 1]
|
| [http://2010.igem.org/UTDallas/2_August_2010 2]
| [http://2010.igem.org/UTDallas/3_August_2010 3]
| [http://2010.igem.org/UTDallas/4_August_2010 4]
| [http://2010.igem.org/UTDallas/5_August_2010 5]
| [http://2010.igem.org/UTDallas/6_August_2010 6]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/7_August_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/8_August_2010&action=edit 8]
|
| [http://2010.igem.org/UTDallas/9_August_2010 9]
| [http://2010.igem.org/UTDallas/10_August_2010 10]
| [http://2010.igem.org/UTDallas/11_August_2010 11]
| [http://2010.igem.org/UTDallas/12_August_2010 12]
| [http://2010.igem.org/UTDallas/13_August_2010 13]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/14_August_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/15_August_2010&action=edit 15]
|
| [http://2010.igem.org/UTDallas/16_August_2010 16]
| [http://2010.igem.org/UTDallas/17_August_2010 17]
| [http://2010.igem.org/UTDallas/18_August_2010 18]
| [http://2010.igem.org/UTDallas/19_August_2010 19]
| [http://2010.igem.org/UTDallas/20_August_2010 20]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/21_August_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/22_August_2010&action=edit 22]
|
| [http://2010.igem.org/UTDallas/23_August_2010 23]
| [http://2010.igem.org/UTDallas/24_August_2010 24]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/25_August_2010&action=edit 25]
| [http://2010.igem.org/UTDallas/26_August_2010 26]
| [http://2010.igem.org/UTDallas/27_August_2010 27]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/28_August_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/29_August_2010&action=edit 29]
|
| [http://2010.igem.org/UTDallas/30_August_2010 30]
| [http://2010.igem.org/UTDallas/31_August_2010 31]
|
|
| September
|
| M | T | W | T | F | S | S
|
|
|
| [http://2010.igem.org/UTDallas/1_September_2010 1]
| [http://2010.igem.org/UTDallas/2_September_2010 2]
| [http://2010.igem.org/UTDallas/3_September_2010 3]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/4_September_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/5_September_2010&action=edit 5]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/6_September_2010&action=edit 6]
| [http://2010.igem.org/UTDallas/7_September_2010 7]
| [http://2010.igem.org/UTDallas/8_September_2010 8]
| [http://2010.igem.org/UTDallas/9_September_2010 9]
| [http://2010.igem.org/UTDallas/10_September_2010 10]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/11_September_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/12_September_2010&action=edit 12]
|
| [http://2010.igem.org/UTDallas/13_September_2010 13]
| [http://2010.igem.org/UTDallas/14_September_2010 14]
| [http://2010.igem.org/UTDallas/15_September_2010 15]
| [http://2010.igem.org/UTDallas/16_September_2010 16]
| [http://2010.igem.org/UTDallas/17_September_2010 17]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/18_September_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/19_September_2010&action=edit 19]
|
| [http://2010.igem.org/UTDallas/20_September_2010 20]
| [http://2010.igem.org/UTDallas/21_September_2010 21]
| [http://2010.igem.org/UTDallas/22_September_2010 22]
| [http://2010.igem.org/UTDallas/23_September_2010 23]
| [http://2010.igem.org/UTDallas/24_September_2010 24]
| [http://2010.igem.org/UTDallas/25_September_2010 25]
| [http://2010.igem.org/UTDallas/26_September_2010 26]
|
| [http://2010.igem.org/UTDallas/27_September_2010 27]
| [http://2010.igem.org/UTDallas/28_September_2010 28]
| [http://2010.igem.org/UTDallas/29_September_2010 29]
| [http://2010.igem.org/UTDallas/30_September_2010 30]
|
|
| October
|
| M | T | W | T | F | S | S
|
|
|
|
|
| [http://2010.igem.org/UTDallas/1_October_2010 1]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/2_October_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/3_October_2010&action=edit 3]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/4_October_2010&action=edit 4]
| [http://2010.igem.org/UTDallas/5_October_2010 5]
| [http://2010.igem.org/UTDallas/6_October_2010 6]
| [http://2010.igem.org/UTDallas/7_October_2010 7]
| [http://2010.igem.org/UTDallas/8_October_2010 8]
| [http://2010.igem.org/UTDallas/9_October_2010 9]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/10_October_2010&action=edit 10]
|
| [http://2010.igem.org/UTDallas/11_October_2010 11]
| [http://2010.igem.org/UTDallas/12_October_2010 12]
| [http://2010.igem.org/UTDallas/13_October_2010 13]
| [http://2010.igem.org/UTDallas/14_October_2010 14]
| [http://2010.igem.org/UTDallas/15_October_2010 15]
| [http://2010.igem.org/UTDallas/16_October_2010 16]
| [http://2010.igem.org/UTDallas/17_October_2010 17]
|
| [http://2010.igem.org/UTDallas/18_October_2010 18]
| [http://2010.igem.org/UTDallas/19_October_2010 19]
| [http://2010.igem.org/UTDallas/20_October_2010 20]
| [http://2010.igem.org/UTDallas/21_October_2010 21]
| [http://2010.igem.org/UTDallas/22_October_2010 22]
| [http://2010.igem.org/UTDallas/23_October_2010 23]
| [http://2010.igem.org/UTDallas/24_October_2010 24]
|
| [http://2010.igem.org/UTDallas/25_October_2010 25]
| [http://2010.igem.org/UTDallas/26_October_2010 26]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/27_October_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/28_October_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/29_October_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/30_October_2010&action=edit 30]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/31_October_2010&action=edit 31]
|
|
| November
|
| M | T | W | T | F | S | S
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/1_November_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/2_November_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/3_November_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/4_November_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/5_November_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/6_November_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/7_November_2010&action=edit 7]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/8_November_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/9_November_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/10_November_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/11_November_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/12_November_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/13_November_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/14_November_2010&action=edit 14]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/15_November_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/16_November_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/17_November_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/18_November_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/19_November_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/20_November_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/21_November_2010&action=edit 21]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/22_November_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/23_November_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/24_November_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/25_November_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/26_November_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/27_November_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/28_November_2010&action=edit 28]
|
| [http://2010.igem.org/wiki/index.php?title=UTDallas/29_November_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=UTDallas/30_November_2010&action=edit 30]
|
|
July 30, 2010
Team Meeting Today!
After eating our pizza, we started discussing the separate components of the biosensor.
First, we discussed the lab work results over the past couple of days with low yields and differing colors. We figured out it was because of the different E.coli strains of DH5α and MG155 used. Instead of using the MG155 used in the last step, we were supposed to use the high efficiency DH5α strain in the transformations to obtain DNA strain.
Plan for Monday: Retransform all of the plasmids.
AROMATICS/NITRATES
Next we discussed the aromatic compounds/nitrate sensor components. We went through the plan that Kristina sent including the possible logic gates. We went through the plasmids of Pr + XylR and the PyeaR promoters including the backbone of pSB1A2. In addition, we discussed the logic gates of the aromatics and the nitrates.
ALKANES
Sameer presented his plan on the alkane hydroxylase with luciferase plan along with his idea about the oxidation of cylcloalkanes instead of the alkanes because of the large size of the allkane parts (35 kbp).
UTD LOGO
In addition, as a supplement to our main project on petroleum sensing, we are conducting a mini-project on producing a UTD logo with colored bacteria based on a photo-sensitive system. LP brought up the idea of not only a red-light activated system, but also creating a signaling regulatory network as either a positive or negative feedback. This connects to our project because of the idea of increased signaling in a controlled system.
Plan for Future: After independent research on all of the parts, we can now join together the ideas into a cohesive whole in our plan for the iGEM project.
"
