"
Team:Newcastle/23 June 2010
From 2010.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
'''23 June 2010''' | '''23 June 2010''' | ||
| - | + | =Wednesday Morning= | |
| - | + | ==Aims== | |
The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone. | The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone. | ||
| - | + | ==Materials== | |
* ''E.coli'' broth culture | * ''E.coli'' broth culture | ||
* Pipettes | * Pipettes | ||
| Line 14: | Line 14: | ||
* Conical flasks | * Conical flasks | ||
| - | + | ==Protocol== | |
Cells are made competent ready for transformation. | Cells are made competent ready for transformation. | ||
| Line 27: | Line 27: | ||
*Positive add 1 μl uncut | *Positive add 1 μl uncut | ||
| - | + | = Wednesday afternoon - taking a break at the Hoppings!!! = | |
| - | + | ==Aims== | |
The aim of this Lab practice was to have lots of fun! | The aim of this Lab practice was to have lots of fun! | ||
| - | + | ==Materials== | |
* Candy floss | * Candy floss | ||
* Suntan lotion | * Suntan lotion | ||
Revision as of 10:41, 11 August 2010
| |||||||||||||
| |||||||||||||
23 June 2010
Contents |
Wednesday Morning
Aims
The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.
Materials
- E.coli broth culture
- Pipettes
- Plasmid ligation products from yesterday
- Conical flasks
Protocol
Cells are made competent ready for transformation.
- Positive control(plasmid uncut)- to make sure cells are transfroming properly
- Negative control (just cells)- nothing but competent cells
5 microfuge tubes:
- 1:5
- 1:3
- Vector
- Negative add nothing
- Positive add 1 μl uncut
Wednesday afternoon - taking a break at the Hoppings!!!
Aims
The aim of this Lab practice was to have lots of fun!
Materials
- Candy floss
- Suntan lotion
- Teddy bears
- Burgers and bacon
- Chips and gravy
- Icecream
- Meerkat toys
- Madmouse ride!
- Beer, wine, lemonade
- Football
| 
| 
|
 |  |  |  |
 |  |  |  |
 |  |  |  |
 |  |  |  |
 |  |  |  |
   
|
    
|
