Team:Newcastle/15 July 2010
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==Aims== | ==Aims== | ||
*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP). | *To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP). | ||
- | * | + | |
+ | ==Materials== | ||
+ | *[[Team:Newcastle/14_July_2010|Second round of Transformants]] | ||
+ | *[[Team:Newcastle/12_July_2010|Plasmid extracts]] | ||
+ | *Restriction enzymes ''Xba''1 aand ''Pst''1 | ||
==Protocol== | ==Protocol== | ||
- | + | Single and Double digest of Plasmid extracts (single ''Xba''1) | |
- | + | Run digests on a gel | |
- | + | Overnight culture of second round of transformants | |
- | + | ||
- | + | ||
- | + | ||
+ | ==Results== | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Revision as of 12:17, 10 August 2010
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Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
Materials
- Second round of Transformants
- Plasmid extracts
- Restriction enzymes Xba1 aand Pst1
Protocol
Single and Double digest of Plasmid extracts (single Xba1) Run digests on a gel Overnight culture of second round of transformants