Team:VT-ENSIMAG/Genothreat

From 2010.igem.org

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=General algorithm=
=General algorithm=
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Following the federal guideline, the first step is to do the six frame translation ([[Team:VT_Ensimag_2010-Biosecurity/sixframe|See More]]) in order to have the corresponding amino-acid strands. We also keep the initial nucleotid strand and its reversed DNA sequence ([[/Why?]]). So we have now 8 sequences to screen (6 amino-acid, 2 nucleotides).
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Following the federal guideline, the first step is to do the six frame translation ([[Team:VT_Ensimag_2010-Biosecurity/sixframe|See More]]) in order to have the corresponding amino-acid strands. We also keep the initial nucleotid strand and its reversed DNA sequence ([[Team:VT_Ensimag_2010_Biosecurity/Why?]]). So we have now 8 sequences to screen (6 amino-acid, 2 nucleotides).
Then, we divide each sequence in 200bps nucleotide or 66 amino-acid subsequences.
Then, we divide each sequence in 200bps nucleotide or 66 amino-acid subsequences.
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Revision as of 21:05, 9 August 2010


VT-ENSIMAG over VT campus long.png

GenoTHREAT




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Home

Our team

Sequence screening

The software: GenoTHREAT

Tests and Results

Screening of the iGEM registry

PCR fusion primer

Lab notebook

Safety

Media Links

Comments

SAIC.jpeg

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Global presentation

Compdna.png

The software, GenoTHREAT takes a DNA sequence on input, and in return told the user if it's a dangerous sequence which need further investigation or if the sequence can be synthetised. It follows the algorithm given by the federal guidelines, and so uses the Blast software for sequence alignement. For managing the sequence (six-frame translation, reversed frame, extracting sub-sequence), we use Biojava, a bio-informatics toolbox ([http://www.biojava.org/wiki/Main_Page 1]). GenoTHREAT has been coded in Java, and is working on both Windows and Linux distribution. We have developped different versions in order to optimize the time execution or the cpus' utilisation according to the use (#Different implementations).

General algorithm

Following the federal guideline, the first step is to do the six frame translation (See More) in order to have the corresponding amino-acid strands. We also keep the initial nucleotid strand and its reversed DNA sequence (Team:VT_Ensimag_2010_Biosecurity/Why?). So we have now 8 sequences to screen (6 amino-acid, 2 nucleotides). Then, we divide each sequence in 200bps nucleotide or 66 amino-acid subsequences.
Each sub-sequence is then blasted, and we extract the relevant information from the blast output. For each sub-sequence, we have to decide if it's a hit or not (See More TODO).

VTENSI Algo.jpg

If one sub-sequence is a hit for our algorithm, then it means that a 200bps sequence potentially dangerous has been detected. The sequence will be so flagged, and a result file containing all the select agent found and some more information will be created.

Different options

Around this algorithm, we have developed different options to use the sequence screening. We can use it to just screen one sequence, or a group of sequence from our test database, or screen sequence from the iGEM registry or GenoCAD database. The parameter on some versions can also be changed in creating a new set of parameters.

Different implementations

Our main issue in implementing the software is the ressources-consuming(time and computational ressources) of blast. In order to get rid of it, we have tried different implementation of our algorithm. See the description and comparison in Different implementations.

Try GenoTHREAT and demonstration

sofware available? demonstration video?

ALIEN DNA.png
VT-ENSIMAG logo.png
ALIEN DNA.png