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Team:Newcastle/9 August 2010
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* For pVeg, we will set up an [[Team:Newcastle/Growing_an_overnight_cultures|overnight broth culture]] from the colonies for [[Team:Newcastle/Qiagen_Minipreps|plasmid extraction]] | * For pVeg, we will set up an [[Team:Newcastle/Growing_an_overnight_cultures|overnight broth culture]] from the colonies for [[Team:Newcastle/Qiagen_Minipreps|plasmid extraction]] | ||
* To show that we have sucessfully transformed from ''lacI'', we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures. | * To show that we have sucessfully transformed from ''lacI'', we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures. | ||
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| + | ==Conclusion== | ||
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| + | Please refer to [[Team:Newcastle/10_August_2010|10.08.10]]. | ||
=Transformation of lacI= | =Transformation of lacI= | ||
Revision as of 10:56, 9 August 2010
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Contents |
Transformation of pVeg and lacI
Results/Aims
- There are colonies on all 3 plates for pVeg.
- For lacI, there is only one colony on one of the normal plates and more on the concentrated one.
Discussion
- For pVeg, we will set up an overnight broth culture from the colonies for plasmid extraction
- To show that we have sucessfully transformed from lacI, we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
Conclusion
Please refer to 10.08.10.
Transformation of lacI
Aims
To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.
Materials and Protocol
Please refer to Transformation of E.coli.
Result
Please refer to 10.08.10 for result.
   
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