"
Team:Newcastle/9 August 2010
From 2010.igem.org
(Difference between revisions)
(→Results/Aims) |
(→Results/Aims) |
||
| Line 6: | Line 6: | ||
* pVeg was successfully transformed - From this we will set up an overnight broth culture for plasmid extraction | * pVeg was successfully transformed - From this we will set up an overnight broth culture for plasmid extraction | ||
| - | * ''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures. | + | * ''lacI'' - To show that we have sucessfully transformed from ''lacI'' we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures. |
=Transformation of lacI= | =Transformation of lacI= | ||
Revision as of 10:30, 9 August 2010
| |||||||||||||
| |||||||||||||
Contents |
Transformation
Results/Aims
- pVeg was successfully transformed - From this we will set up an overnight broth culture for plasmid extraction
- lacI - To show that we have sucessfully transformed from lacI we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
Transformation of lacI
Aims
To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.
Materials and Protocol
Please refer to Transformation of E.coli.
Result
Please refer to 10.08.10 for result.
   
|
    
|
