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Team:Newcastle/9 August 2010
From 2010.igem.org
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=Transformation= | =Transformation= | ||
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==Results/Aims== | ==Results/Aims== | ||
*pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction | *pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction | ||
*''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture. | *''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture. | ||
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=Transformation from ligation products= | =Transformation from ligation products= | ||
Revision as of 10:15, 9 August 2010
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Contents |
Transformation
Results/Aims
- pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
- lacI- To show that we have sucessfully transformed from lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
Transformation from ligation products
Aims
To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.
   
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