"
Team:Newcastle/9 August 2010
From 2010.igem.org
(Difference between revisions)
(→Ligation) |
(→Aims) |
||
| Line 11: | Line 11: | ||
==Aims== | ==Aims== | ||
| - | Today we will transform some competent ''E.coli'' | + | Today we will transform some competent ''E.coli'' DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on [[Team:Newcastle/6_August_2010|06.08.10]]. |
| + | |||
| + | =Transformation from ligation products= | ||
| + | |||
| + | ==Aims== | ||
| + | |||
| + | To transform some competent ''E.coli'' DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on [[Team:Newcastle/6_August_2010|06.08.10]]. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 10:14, 9 August 2010
| |||||||||||||
| |||||||||||||
Contents |
Transformation
Results/Aims
- pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
- lacI- To show that we have sucessfully transformed from lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
Ligation
Aims
Today we will transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.
Transformation from ligation products
Aims
To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.
   
|
    
|
