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Team:Newcastle/15 July 2010
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| + | =LacI BioBrick Construction= | ||
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| + | ==Aims== | ||
| + | *To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP). | ||
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Revision as of 13:15, 6 August 2010
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LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
Screening for lacI insert:
- (Miniprep)
- Restriction digests - the restriction digest protocol was followed.
- Single digest with Pst1
- double digests with Pst1 and Xba1
- Gel electrophoresis
Set up liquid broth culture in 5 ml of LB - 1 colony from colonies 1-7
   
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