Team:TU Delft/9 July 2010 content

From 2010.igem.org

(Difference between revisions)
(Ordered DNA stocks)
 
Line 1: Line 1:
=Lab work=
=Lab work=
==Ordered DNA stocks==
==Ordered DNA stocks==
-
Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 DNA parts].
+
Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered [https://2010.igem.org/Team:TU_Delft#page=pages/blog&blog=6_July_2010 DNA parts].
The following plasmid concentrations were obtained:
The following plasmid concentrations were obtained:

Latest revision as of 12:37, 3 August 2010

Lab work

Ordered DNA stocks

Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered DNA parts.

The following plasmid concentrations were obtained:

DNA part Concentration (ng/μL)
alkB2 147.3
ruBA3 63.6
rubA4 29.0
rubR 26.7
ladA 5011.2
ADH 178.8
ALDH 24.1
bbc1 5.5
AlnA 357.4
OprG 197.3
AlkS 510.8
PalkB 61.5
PalkS1-2 60.9
P(Caif) 9.7
PhPFDα 147.0
PhPFDβ 56.7

rubA4, rubR, ALDH, bbc1 and P(Caif) have a low concentration, so we going to repeat the plasmid isolation of these samples.