Team:TU Delft/9 July 2010 content

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==Lab work==
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=Lab work=
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<h4>Ordered DNA stocks</h4>
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==Ordered DNA stocks==
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Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 DNA parts].
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Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered [https://2010.igem.org/Team:TU_Delft#page=pages/blog&blog=6_July_2010 DNA parts].
The following plasmid concentrations were obtained:
The following plasmid concentrations were obtained:
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|rubA4
|rubA4
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|29.0
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|rubR
|rubR
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|26.7
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|ladA
|ladA
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|5011.2
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|ADH
|ADH
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|178.8
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|ALDH
|ALDH
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|24.1
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|bbc1
|bbc1
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|5.5
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|AlnA
|AlnA
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|357.4
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|OprG
|OprG
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|197.3
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|AlkS
|AlkS
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|510.8
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|PalkB
|PalkB
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|61.5
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|PalkS1-2
|PalkS1-2
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|60.9
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|P(Caif)
|P(Caif)
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|9.7
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|PhPFDα
|PhPFDα
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|147.0
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|PhPFDβ
|PhPFDβ
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|56.7
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rubA4, rubR, ALDH, bbc1 and P(Caif) have a low concentration, so we going to repeat the plasmid isolation of these samples.

Latest revision as of 12:37, 3 August 2010

Lab work

Ordered DNA stocks

Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered DNA parts.

The following plasmid concentrations were obtained:

DNA part Concentration (ng/μL)
alkB2 147.3
ruBA3 63.6
rubA4 29.0
rubR 26.7
ladA 5011.2
ADH 178.8
ALDH 24.1
bbc1 5.5
AlnA 357.4
OprG 197.3
AlkS 510.8
PalkB 61.5
PalkS1-2 60.9
P(Caif) 9.7
PhPFDα 147.0
PhPFDβ 56.7

rubA4, rubR, ALDH, bbc1 and P(Caif) have a low concentration, so we going to repeat the plasmid isolation of these samples.

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