Team:TU Delft/9 July 2010 content

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(New page: ===Lab work=== Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 DNA parts].)
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===Lab work===
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=Lab work=
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Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 DNA parts].
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==Ordered DNA stocks==
 +
Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered [https://2010.igem.org/Team:TU_Delft#page=pages/blog&blog=6_July_2010 DNA parts].
 +
 
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The following plasmid concentrations were obtained:
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
 +
|'''DNA part'''
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|'''Concentration (ng/μL)'''
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|-
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|alkB2
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|147.3
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|-
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|ruBA3
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|63.6
 +
|-
 +
|rubA4
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|29.0
 +
|-
 +
|rubR
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|26.7
 +
|-
 +
|ladA
 +
|5011.2
 +
|-
 +
|ADH
 +
|178.8
 +
|-
 +
|ALDH
 +
|24.1
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|-
 +
|bbc1
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|5.5
 +
|-
 +
|AlnA
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|357.4
 +
|-
 +
|OprG
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|197.3
 +
|-
 +
|AlkS
 +
|510.8
 +
|-
 +
|PalkB
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|61.5
 +
|-
 +
|PalkS1-2
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|60.9
 +
|-
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|P(Caif)
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|9.7
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|-
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|PhPFDα
 +
|147.0
 +
|-
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|PhPFDβ
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|56.7
 +
|}
 +
 
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rubA4, rubR, ALDH, bbc1 and P(Caif) have a low concentration, so we going to repeat the plasmid isolation of these samples.

Latest revision as of 12:37, 3 August 2010

Lab work

Ordered DNA stocks

Ramon and Eva are performing Qiagen Maxi-prep plasmid isolation of ordered DNA parts.

The following plasmid concentrations were obtained:

DNA part Concentration (ng/μL)
alkB2 147.3
ruBA3 63.6
rubA4 29.0
rubR 26.7
ladA 5011.2
ADH 178.8
ALDH 24.1
bbc1 5.5
AlnA 357.4
OprG 197.3
AlkS 510.8
PalkB 61.5
PalkS1-2 60.9
P(Caif) 9.7
PhPFDα 147.0
PhPFDβ 56.7

rubA4, rubR, ALDH, bbc1 and P(Caif) have a low concentration, so we going to repeat the plasmid isolation of these samples.

Retrieved from "http://2010.igem.org/Team:TU_Delft/9_July_2010_content"