Friday July 30, 2010

Chris's first gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50°C to 56°C and at a MgCl₂ concentration of 1.5 µM.

Chris's second gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50°C to 56°C and at a MgCl₂ concentration of 2.0 µM.

Chris's third gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50°C to 56°C and at a MgCl₂ concentration of 2.5 µM

Himika

Today there was a meeting which updated updates all the team members about each other. I also kept looking into MATLAB tutorials and tried out some of the tutorials. I also read some of the papers about inclusion bodies. This will be used to model of the project. The sequence data of the I0500-B0034 came back today and it was successful it seems. So I have decided to go forth with the construct and add on more parts next week.

Chris

Today, I did the minipreps for the constructions of I0500-I13504 and I0500-I13507 using the Sigma Aldrich Plasmid Preparation kit. As well, I ran 3 gels of the gradient PCR which removed the CpxP promoter from the E. coli genome. The three different lines were mixed together so there are three tubes of PCR product instead of 36. Finally, Henry gave us instruments which would allow for the vacuum separation of PCR product from contaminants.