Revision as of 09:15, 30 July 2010

Gel electrophoresis

Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview dye.
Wait for 30 min to allow the gel to harden.
Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge.
Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA.
Loading buffer was then added together with the sample before loading onto the gel matrix.
Run gel at 90V until separation is achieved and visualize using the gelDoc.

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@@ Line 12: / Line 12: @@
 ==Procedures==
-# Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview
+# Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview dye.
-# Wait for 30 min to allow the gel to harden
+# Wait for 30 min to allow the gel to harden.
-# Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge
+# Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge.
-# Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA
+# Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA.
-# Loading buffer was then added together with the sample before loading onto the gel matrix
+# Loading buffer was then added together with the sample before loading onto the gel matrix.
-# Run gel at 90V until separation is achieved and visualize using the gelDoc
+# Run gel at 90V until separation is achieved and visualize using the gelDoc.
+'''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]'''
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