Making Media

From 2010.igem.org

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   * 5g glucose
   * 5g glucose
   * 10ml Deionized Water
   * 10ml Deionized Water
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Latest revision as of 19:58, 29 July 2010

LB Media (RT)

  * 10g Tryptone
  * 10g NaCl
  * 5g Yeast Extract
  * 1L Deionized Water
  

SOB Media (RT)

  * 20g Tryptone 
  * 5g Yeast Extract
  * 0.5g NaCl
  * 0.2g KCl
  * 1L Deionized Water


SOC Media (4 degrees Celsius)

  * Add the following per ml SOB:
      - 4ul MgCl2 Solution (100)
      - 8ul Glucose Solution (200)


Glucose Stock Solution (4 degrees Celsius)

  * 5g glucose
  * 10ml Deionized Water





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