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Revision as of 18:58, 19 July 2010 (view source) Anisha (Talk | contribs) (New page: '''Daily Schedule''' 1. Miniprep Flasks (1 hr) 2. Restriction Digest (1 hr) A)Standard Assembly i.Small part EX or SP digest ii.Large Part XP or ES digest a...) ← Older edit		Latest revision as of 19:57, 29 July 2010 (view source) Erik (Talk | contribs)
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		+	[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook]

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Latest revision as of 19:57, 29 July 2010

Daily Schedule

1. Miniprep Flasks (1 hr)

2. Restriction Digest (1 hr)

       A)Standard Assembly
          i.Small part EX or SP digest
          ii.Large Part XP or ES digest and Phosphotase (1 hr)
       B)3-A Assembly
          i.Part 1 ES digest
          ii.Part 2 XP digest
          iii.Backbone EP digest (Phosphotase if we want)
       C)During digestion run a colony PCR of yesterday’s plates

3. Run Gel (1.5 hrs)

       A)Include PCR products on gel

4. Ligation (1/2 hr)

5. Transformation (1/2 hr)

6. Cell Recovery (1 hr)

       A)During recovery send colony PCR results to the sequencing center

7. Plate Ligation

Return to Notebook