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Team:Newcastle/Growing an overnight cultures
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| - | + | =Growing an Overnight Culture= | |
| - | + | ==Materials Required== | |
| + | |||
| + | * LB Broth | ||
| + | * Colonies of bacteria | ||
| + | * Flame loop | ||
| + | * Universal tube | ||
| + | * Pipette | ||
| + | |||
| + | ==Procedures== | ||
| + | |||
| + | # 5ml of LB broth is pipetted into the universal tube. | ||
| + | # The flame loop is used to pick a colony. | ||
| + | # The colony is then mixed into the LB Broth in the universal tube. | ||
| + | # The culture is left overnight at 37°C (''E.coli'', ''B.subtilis'' lower temp) | ||
| + | |||
| + | |||
| + | {{Team:Newcastle/footer}} | ||
Revision as of 15:05, 29 July 2010
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Growing an Overnight Culture
Materials Required
- LB Broth
- Colonies of bacteria
- Flame loop
- Universal tube
- Pipette
Procedures
- 5ml of LB broth is pipetted into the universal tube.
- The flame loop is used to pick a colony.
- The colony is then mixed into the LB Broth in the universal tube.
- The culture is left overnight at 37°C (E.coli, B.subtilis lower temp)
   
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