Team:Harvard/color/notebook
From 2010.igem.org
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+ | <span id="date"><a name="07-23-2010" class="date">07-23-2010</a></span> [<a class="top" href="#notebook">top</a>]<hr /> | ||
+ | <ul> | ||
+ | <li>Received primers for LUT2 and BETA-OHASE 1 - began building knockdown constructs</li> | ||
+ | <li>PCR reaction on RS300 to insert recognition sites. Primers listed below</li> | ||
+ | <li class="indent">LUT2 rxn 1: A, CP4</li> | ||
+ | <li class="indent">LUT2 rxn 2: CP2, CP3</li> | ||
+ | <li class="indent">LUT2 rxn 3: CP1, B</li> | ||
+ | <li class="indent">BETA-OHASE 1 rxn 1: A, CP8</li> | ||
+ | <li class="indent">BETA-OHASE 1 rxn 2: CP6, CP7</li> | ||
+ | <li class="indent">BETA-OHASE 1 rxn 3: CP5, B</li> | ||
+ | <li>PCR purified finished reaction and ran 4 ul on a 1% agarose gel</li> | ||
+ | <div><a href="https://static.igem.org/mediawiki/2010/0/0b/072610amipcr1_ann.png" id="single_image"><img src="https://static.igem.org/mediawiki/2010/0/0b/072610amipcr1_ann.png" width="100px"/> [click to enlarge]</a></div> | ||
+ | <li>Cut out backbone (top) bands from O1, O2, R1 and saved them at 4°C </li></ul> | ||
+ | </div> | ||
</html> | </html> |
Revision as of 19:04, 28 July 2010
![](https://static.igem.org/mediawiki/2010/9/93/Harvard2010colorbanner.png)
notebook
Week 6 | - | - | - | - | 07-23-2010 |
Week 7 | 07-26-2010 | 07-27-2010 | 07-28-2010 | 07-29-2010 | 07-30-2010 |
07-23-2010 [top]
- Received primers for LUT2 and BETA-OHASE 1 - began building knockdown constructs
- PCR reaction on RS300 to insert recognition sites. Primers listed below
- LUT2 rxn 1: A, CP4
- LUT2 rxn 2: CP2, CP3
- LUT2 rxn 3: CP1, B
- BETA-OHASE 1 rxn 1: A, CP8
- BETA-OHASE 1 rxn 2: CP6, CP7
- BETA-OHASE 1 rxn 3: CP5, B
- PCR purified finished reaction and ran 4 ul on a 1% agarose gel
- Cut out backbone (top) bands from O1, O2, R1 and saved them at 4°C