Team:Newcastle/Gel electrophoresis

Gel electrophoresis

Materials

• 1X TAE buffer
• SafeView
• Agarose
• Eppendorf
• Gel making tank
• Gel running tank

Procedures

1. Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview
2. Wait for 30 min to allow the gel to harden
3. Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge
4. Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA
5. Loading buffer was then added together with the sample before loading onto the gel matrix
6. Run gel at 90V until separation is achieved and visualize using the gelDoc