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Team:Newcastle/Gel electrophoresis
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RachelBoyd (Talk | contribs) (New page: {{Team:Newcastle/mainbanner}} ===Gel electrophoresis=== *Transfer harden gel into the gel tank *add TAE buffer until the gel is completely submerged *Depending on the nature of the samp...) |
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===Gel electrophoresis=== | ===Gel electrophoresis=== | ||
| - | *Transfer harden gel into the gel tank | + | ==Materials== |
| - | + | * | |
| - | *Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder | + | |
| - | *Loading buffer was then added together with the sample before loading onto the gel matrix | + | ==Procedures== |
| - | *Run gel at 90V until separation is achieved and visualize using the gelDoc | + | * Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview |
| + | * Wait for 30 min to allow the gel to harden | ||
| + | * Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge | ||
| + | * Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA | ||
| + | * Loading buffer was then added together with the sample before loading onto the gel matrix | ||
| + | * Run gel at 90V until separation is achieved and visualize using the gelDoc | ||
Revision as of 14:36, 28 July 2010
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Gel electrophoresis
Materials
Procedures
- Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview
- Wait for 30 min to allow the gel to harden
- Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge
- Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA
- Loading buffer was then added together with the sample before loading onto the gel matrix
- Run gel at 90V until separation is achieved and visualize using the gelDoc
