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Team:Newcastle/2 July 2010
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The aim of this experiment was to determine if ''Bacillus subtilis'' 168 is able to produce urease and degrade urea. | The aim of this experiment was to determine if ''Bacillus subtilis'' 168 is able to produce urease and degrade urea. | ||
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The negative control did not turn pink-red color, therefore indicating that no contamination had occurred. | The negative control did not turn pink-red color, therefore indicating that no contamination had occurred. | ||
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| + | * Produce [[Team:Newcastle/E. coli Competence|competent ''E. coli'']] (DH5alpha strain) | ||
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Revision as of 10:13, 27 July 2010
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Contents |
Aims
The aim of this experiment was to determine if Bacillus subtilis 168 is able to produce urease and degrade urea.
Urease test
The experiment was performed on 01.07.10. For the experimental protocol, please see 01.07.10 lab notebook.
Results
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Figure 1: Urease test results
- Negative control - No color change (Orange color)
- Test 1 (Duplicate) - Pink-red color
- Test 2 (Duplicate) - Pink-red color
Conclusion
B. subtilis 168 is able to produce urease, therefore, urea which is the substrate and can be found in the agar was degraded, which results in ammonia building. The ammonia makes the media alkaline and therefore the indicator phenol red will change from orange color to pink-red color.
The negative control did not turn pink-red color, therefore indicating that no contamination had occurred.
- Produce competent E. coli (DH5alpha strain)
   
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