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Team:Cambridge/References/ProjectBioluminescence/Experiments
From 2010.igem.org
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==2. Test use of CHBT as a substrate== | ==2. Test use of CHBT as a substrate== | ||
| - | + | '''a) D-cysteine''' | |
| - | ** | + | With sucessfully transformed cells from 1 compare emission when adding: |
| + | ** D-luciferin | ||
| + | ** CHBT | ||
| + | ** CHBT + D-Cysteine | ||
| + | ** CHBT + L-Cysteine | ||
| + | ** (CHBT + L-Luciferin? if necessary) | ||
'''b) L-cysteine via L-luciferin''' | '''b) L-cysteine via L-luciferin''' | ||
Revision as of 16:06, 26 July 2010
Firefly
1. Test luminescence with the Luciferase from the registry
- Transform WT E.coli with the luciferase from the registry.
- Feed with D-luciferin, develop a method for measuring light output
- Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
- Controls - no luciferin, no inducer
2. Test use of CHBT as a substrate
a) D-cysteine With sucessfully transformed cells from 1 compare emission when adding:
- D-luciferin
- CHBT
- CHBT + D-Cysteine
- CHBT + L-Cysteine
- (CHBT + L-Luciferin? if necessary)
b) L-cysteine via L-luciferin
