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Team:TU Delft/21 June 2010 content
From 2010.igem.org
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
|'''Digestion reaction''' | |'''Digestion reaction''' | ||
| + | |'''Used Buffe'''r | ||
|'''Needed fragment''' | |'''Needed fragment''' | ||
|- | |- | ||
| - | |1,0 μg pSB1T3 + 1 U/μL EcoRI + 1 U/μL PstI | + | |1,0 μg pSB1T3 + 1 U/μL EcoRI (Roche) + 1 U/μL PstI (Roche) |
| + | |Buffer H (Roche) | ||
|‘E – ---- – P’ | |‘E – ---- – P’ | ||
|} | |} | ||
Revision as of 20:05, 17 July 2010
Lab work
Characterization of Anderson RBS sequences
Restriction digestion of plasmid backbone pSB1T3 using Buffer H of Roche.
| Digestion reaction | Used Buffer | Needed fragment |
| 1,0 μg pSB1T3 + 1 U/μL EcoRI (Roche) + 1 U/μL PstI (Roche) | Buffer H (Roche) | ‘E – ---- – P’ |
The digested pSB1T3 was cut from gel and isolate the band. However, the gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.
