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Team:TU Delft/11 June 2010 content
From 2010.igem.org
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<h4>BioBrick stocks</h4> | <h4>BioBrick stocks</h4> | ||
Today we performed a [[Team:TU_Delft/protocols/mini-prep_plasmid_isolation|Qiagen Mini-prep plasmid isolation]] of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=8_June_2010 vectors] we grew overnight. We shaved 3 mL of the bacterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. | Today we performed a [[Team:TU_Delft/protocols/mini-prep_plasmid_isolation|Qiagen Mini-prep plasmid isolation]] of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=8_June_2010 vectors] we grew overnight. We shaved 3 mL of the bacterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. | ||
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The following plasmid concentrations were obtained: | The following plasmid concentrations were obtained: | ||
| + | [[Image:P1010005_small.jpg|200px|thumb|right|Thias doing labwork]] | ||
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| - | [[Image:P1010002_small.jpg| | + | [[Image:P1010002_small.jpg|250px|thumb|right|Ramon & Hugo in the lab]] |
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Revision as of 17:04, 17 July 2010
Contents |
Lab work
BioBrick stocks
Today we performed a Qiagen Mini-prep plasmid isolation of the vectors we grew overnight. We shaved 3 mL of the bacterial cells to make -80 °C stocks.
The following plasmid concentrations were obtained:
| BioBrick | Concentration (ng/μL) |
| pSB3T5 | 266.0 |
| pSB1T3 | 414.0 |
| pSB1C3 | 284.7 |
| pSB3C5 | 329.3 |
| pSB1K3 | 270.1 |
| pSB1A3 | 271.9 |
| I13401 | 232.1 |
Characterization of Anderson RBS sequences
Next to that we also started digesting the first parts we will be characterizing.
Spring Workshop
Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep.
