Team:Penn State/Project

From 2010.igem.org

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Revision as of 16:47, 15 July 2010


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety



Contents

Overall project

The Penn State Team will be researching and experimenting two different ideas. The first area of study will be creating an oxygen-sensing promoter. This will be done through researching the way that FNR and arcA regulate aerobic/anaerobic respiration in E. coli; engineering applications will be applied to these processes. The second topic we are working on is Bacterial Fireworks, an incorporation of quorum sensing, pigments, and cell lysis. Our goal is to assemble the necessary parts to display the spreading of color as the cells lyse. The team would like to incorporate both aspects of the project to create a bacterial fireworks display that is triggered by a lack of oxygen inside of an agar sphere.


A reliable modular lysis mechanism through quorum sensing could be used in many different fields. As engineered microorganisms are becoming more widely considered for use in the open environment, concerns are growing about possible detrimental effects these microbes could have on ecosystems if they were ever to grow and multiply past expectations. A quorum-based lysis "safety" mechanism would only require one cell to receive a predetermined signal in order to eliminate the entire rogue population, should it escape the area of its intended use.

Project Details

Part 1

The Penn State iGEM team began work and research in mid May. After learning lab techniques from the grad students, we began researching possible topics. We decided to create a cell lysis device and an Oxygen sensing mechanism.


Initially, we transformed Lux Promoters, Constitutive Promoters, LuxI + RBS, LuxR + RBS, Lambda Phage lysis devices, Terminators, LacI, LuxR, pLac, CI, RBS, Pigments, FP, LacI + RBS, and CI + RBS.

Lux Promoters Constitutive Promoters LuxI + RBS LuxR + RBS Lambda Phage Lysis Device Terminator LacI LuxR
K091107 J23108 C0261 J37033 K112022 B0010 C0012 C0062
K091146 J23116 S01414 K112808 B1002
K145150 J23100 B1006
R0061
R0063
R1062



The Experiments

Part 2

Results

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