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| - | <!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** -->
| + | [[Image:mnlogo.jpg|300px|center|frame]] |
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| - | <html>
| + | {| style="color:gold;background-color:#800000;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="90%" align="center" |
| - | <div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
| + | !align="center"|[[Team:Minnesota|<font color="gold">Home</font>]] |
| - | <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
| + | !align="center"|[[Team:Minnesota/Team|<font color="gold">Team</font>]] |
| - | This is a template page. READ THESE INSTRUCTIONS.
| + | !align="center"|[[Team:Minnesota/Project|<font color="gold">Project</font>]] |
| - | </div> | + | !align="center"|[[Team:Minnesota/Protocols|<font color="gold">Protocols</font>]] |
| - | <div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
| + | !align="center"|[[Team:Minnesota/Notebook|<font color="gold">Notebook</font>]] |
| - | You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
| + | !align="center"|[[Team:Minnesota/Judging|<font color="gold">Judging Criteria</font>]] |
| - | </div> | + | !align="center"|[[Team:Minnesota/Attributions|<font color="gold">Attributions</font>]] |
| - | <div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
| + | !align="center"|[[Team:Minnesota/Safety|<font color="gold">Safety</font>]] |
| - | You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
| + | |} |
| - | </div>
| + | {|align="justify" |
| - | </div> | + | <h1>Welcome!</h1> |
| - | </html> | + | Welcome to the Team Minnesota Wiki for iGEM 2010! |
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| - | <!-- *** End of the alert box *** -->
| + | We are a team of undergraduate students along with many faculty and post-doc advisors. This is the third year that Minnesota has sent a team to iGEM. |
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| | + | [[Image:MN Igem10 team.jpg|center]] |
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| | + | Team Minnesota take won a gold medal and the Best Natural Part Special Prize at iGEM 2010!! |
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| - | {|align="justify"
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| - | |You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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| - | |[[Image:Minnesota_logo.png|200px|right|frame]]
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| - | ''Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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| - | |[[Image:Minnesota_team.png|right|frame|Your team picture]]
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| - | |align="center"|[[Team:Minnesota | Team Example]]
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| - | |}
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| - | <!--- The Mission, Experiments ---> | + | <b>Metabolic Engineering: In vivo Nanobioreactors</b> |
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| - | {| style="color:gold;background-color:#800000;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="90%" align="center"
| + | Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. For our iGEM project, we have cloned (and expressed in E. coli) Salmonella LT2 genes responsible for the production and assembly of ethanolamine utilization microcompartments. Additionally, we have determined a signal sequence that targets an ethanolamine utilization enzyme to the microcompartment and verified this by fusing the sequence to GFP and observing that this causes the GFP reporter to localize to the compartment. We conclude that recombinant microcompartments housing targeted enzymes can function as in vivo bioreactors with high reaction efficiencies. |
| - | !align="center"|[[Team:Minnesota|<font color="gold">Home</font>]]
| + | |
| - | !align="center"|[[Team:Minnesota/Team|<font color="gold">The Team</font>]] | + | |
| - | !align="center"|[[Team:Minnesota/Project|<font color="gold">The Project</font>]]
| + | |
| - | !align="center"|[[Team:Minnesota/Designer|<font color="gold">SynBioSS Designer</font>]]
| + | <h2>Highlights for the Judges</h2> |
| - | !align="center"|[[Team:Minnesota/Modeling|<font color="gold">Modeling</font>]]
| + | Judges! Please navigate to our [[Team:Minnesota/Judging|Judging Criteria]] page for a comprehensive list of UMN's fulfilled medal requirements and links throughout the wiki. |
| - | !align="center"|[[Team:Minnesota/Notebook|<font color="gold">Experimental</font>]]
| + | |
| - | !align="center"|[[Team:Minnesota/Parts Characterization|<font color="gold">Competition Requirements</font>]]
| + | {|align="center" cellpadding="25" |
| - | |}
| + | |[[Image:UMNSponsors.png|300px|center|frame|Sponsers]] |
Welcome!
Welcome to the Team Minnesota Wiki for iGEM 2010!
We are a team of undergraduate students along with many faculty and post-doc advisors. This is the third year that Minnesota has sent a team to iGEM.
Team Minnesota take won a gold medal and the Best Natural Part Special Prize at iGEM 2010!!
Metabolic Engineering: In vivo Nanobioreactors
Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. For our iGEM project, we have cloned (and expressed in E. coli) Salmonella LT2 genes responsible for the production and assembly of ethanolamine utilization microcompartments. Additionally, we have determined a signal sequence that targets an ethanolamine utilization enzyme to the microcompartment and verified this by fusing the sequence to GFP and observing that this causes the GFP reporter to localize to the compartment. We conclude that recombinant microcompartments housing targeted enzymes can function as in vivo bioreactors with high reaction efficiencies.
Highlights for the Judges
Judges! Please navigate to our Judging Criteria page for a comprehensive list of UMN's fulfilled medal requirements and links throughout the wiki.
 Sponsers |
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