Team:Heidelberg/Notebook/Material/Primer
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(New page: {{:Team:Heidelberg/Template}} {{:Team:Heidelberg/tables|normal=FFF|highlight=ddd}} {{:Team:Heidelberg/Pagetop|primer}} ==Primer Table== {| border="1" class="wikitable zebra sortable" c...) |
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{{:Team:Heidelberg/tables|normal=FFF|highlight=ddd}} | {{:Team:Heidelberg/tables|normal=FFF|highlight=ddd}} | ||
- | {{:Team:Heidelberg/ | + | {{:Team:Heidelberg/Single_Pagetop|note_primer}} |
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+ | __NOTOC__ | ||
==Primer Table== | ==Primer Table== | ||
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|align="right"| 001 || Casp8FWEcoR1 || ttttgaattcatggacttcagcagaaatc || Caspase8 death gene || Caspase8 || | |align="right"| 001 || Casp8FWEcoR1 || ttttgaattcatggacttcagcagaaatc || Caspase8 death gene || Caspase8 || | ||
|- | |- | ||
- | |align="right"| 002 || CMV_AgeI_fw || ttttaccggtagaatctgcttagggttagg || CMV, shRNA || pcDNA5/FRT-shRNA || | + | |align="right"| 002 || CMV_AgeI_fw || ttttaccggtagaatctgcttagggttagg || CMV, shRNA || pcDNA5/FRT-shRNA || pTR-UF3 |
|- | |- | ||
- | |align="right"| 003 || CMVshRNApA_BglII_rv || taatagatctcagaagccatagagcccacc || CMV, shRNA || pcDNA5/FRT-shRNA || | + | |align="right"| 003 || CMVshRNApA_BglII_rv || taatagatctcagaagccatagagcccacc || CMV, shRNA || pcDNA5/FRT-shRNA || pTR-UF3 |
|- | |- | ||
|align="right"| 004 || EGFPRVFse1 || ttttggccggcccttgtacagctcgtccatg || EGFP || GFP || | |align="right"| 004 || EGFPRVFse1 || ttttggccggcccttgtacagctcgtccatg || EGFP || GFP || | ||
Line 62: | Line 64: | ||
|align="right"| 026 || Hcrrev_ApaI_SalI_TL || ctcgagggcccgtcgacaagcaaacgatgccaagacatttatcg || part 4 shRNA against everything; cutting site: ApaI, SalI || miRsAg || pC-DNA5 | |align="right"| 026 || Hcrrev_ApaI_SalI_TL || ctcgagggcccgtcgacaagcaaacgatgccaagacatttatcg || part 4 shRNA against everything; cutting site: ApaI, SalI || miRsAg || pC-DNA5 | ||
|- | |- | ||
- | |align="right"| 027 || HSVluc_norm_BglII_fw || tttttagatcttgcaggagcttcagggagtg || HSV-TK, ''Firefly'' Luciferase || psiCHECK2 || | + | |align="right"| 027 || HSVluc_norm_BglII_fw || tttttagatcttgcaggagcttcagggagtg || HSV-TK, ''Firefly'' Luciferase || psiCHECK2 || pTR-UF3 |
|- | |- | ||
- | |align="right"| 028 || HSVluc_norm_BglII_rv || ttttagatctggttccgcgcacatttccc || HSV-TK, ''Firefly'' Luciferase || psiCHECK2 || | + | |align="right"| 028 || HSVluc_norm_BglII_rv || ttttagatctggttccgcgcacatttccc || HSV-TK, ''Firefly'' Luciferase || psiCHECK2 || pTR-UF3 |
|- | |- | ||
|align="right"| 029 || Kif11_PLK1_shRNA-hcrfor:ecoRI || gagtcgaattctggaggtgaagttaacaccttcgtg || part 1 shRNA against everything; cutting site: ecoRI || miRsAg || pTR-UF3 | |align="right"| 029 || Kif11_PLK1_shRNA-hcrfor:ecoRI || gagtcgaattctggaggtgaagttaacaccttcgtg || part 1 shRNA against everything; cutting site: ecoRI || miRsAg || pTR-UF3 | ||
Line 88: | Line 90: | ||
|align="right"| 039 || pTR-UF3_PstI-NsiI_shifted_rev || cattaatgcagctgggatgcatccagctggcgtaatagcgaagag || mutagenesis of PstI to NsiI || pTR-UF3 || pTR-UF3 | |align="right"| 039 || pTR-UF3_PstI-NsiI_shifted_rev || cattaatgcagctgggatgcatccagctggcgtaatagcgaagag || mutagenesis of PstI to NsiI || pTR-UF3 || pTR-UF3 | ||
|- | |- | ||
- | |align="right"| 040 || pTRUF3_seq_fwrv || caactccatcactaggggttcct || sequencing purpose || | + | |align="right"| 040 || pTRUF3_seq_fwrv || caactccatcactaggggttcct || sequencing purpose || pTR-UF3 || pTR-UF3 |
|- | |- | ||
|align="right"| 041 || SCLpsv40_FW || ctcaattagtcagcaaccatagtccc || FWprimer for testing stable integration of shRNA into T-Rex || || T-REx cells | |align="right"| 041 || SCLpsv40_FW || ctcaattagtcagcaaccatagtccc || FWprimer for testing stable integration of shRNA into T-Rex || || T-REx cells | ||
Line 96: | Line 98: | ||
|align="right"| 043 || Sequencing primer_pTR-UF3 mutated || ctaaatcggaaccctaaagggagcccccg || sequencing of mutagenesis of PstI to NsiI || pTR-UF3 || pTR-UF3 | |align="right"| 043 || Sequencing primer_pTR-UF3 mutated || ctaaatcggaaccctaaagggagcccccg || sequencing of mutagenesis of PstI to NsiI || pTR-UF3 || pTR-UF3 | ||
|- | |- | ||
- | |align="right"| 044 || Sv40lucmcs_AgeI_fw || ttttaccggtgtggaatgtgtgtcagttag || SV40, ''Renilla'' Luciferase, MCS || | + | |align="right"| 044 || Sv40lucmcs_AgeI_fw || ttttaccggtgtggaatgtgtgtcagttag || SV40, ''Renilla'' Luciferase, MCS || pTR-UF3 || |
|- | |- | ||
- | |align="right"| 045 || Sv40lucmcs_BglII_rv || tattagatctccgcgtcagacaaaccctaac || SV40, ''Renilla'' Luciferase, MCS || psiCHECK2 || | + | |align="right"| 045 || Sv40lucmcs_BglII_rv || tattagatctccgcgtcagacaaaccctaac || SV40, ''Renilla'' Luciferase, MCS || psiCHECK2 || pTR-UF3 |
|- | |- | ||
- | |align="right"| 046 || tBidGFPRVFse1 || tttttggccggccttacatgtacagctcg || tBid death gene || | + | |align="right"| 046 || tBidGFPRVFse1 || tttttggccggccttacatgtacagctcg || tBid death gene || pcherryBidGFP || |
|- | |- | ||
- | |align="right"| 047 || tBidFWEcoR1 || tttttgaattcaaccgcagcagccactc || tBid death gene || | + | |align="right"| 047 || tBidFWEcoR1 || tttttgaattcaaccgcagcagccactc || tBid death gene || pcherryBidGFP || |
|- | |- | ||
- | |align="right"| 048 || tBidRVFse1 || tttttccggccgggtccatcccatttctg || tBid death gene || | + | |align="right"| 048 || tBidRVFse1 || tttttccggccgggtccatcccatttctg || tBid death gene || pcherryBidGFP || |
|- | |- | ||
|align="right"| 049 || TetO2FW || ggggacaacttttctatacaaagttgtccctatcagtgatagagatctc || TetO8 || synthesized tetO8 || Gateway entry vector | |align="right"| 049 || TetO2FW || ggggacaacttttctatacaaagttgtccctatcagtgatagagatctc || TetO8 || synthesized tetO8 || Gateway entry vector | ||
|} | |} | ||
- | |||
- | |||
==Standart Kit Cloning Primers== | ==Standart Kit Cloning Primers== | ||
- | |||
{| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | {| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | ||
- | !align="right"| ID !! Name !! Sequence (5' to 3') | + | !align="right"| ID !! Name !! Sequence (5' to 3') |
|- | |- | ||
- | |align="right"| D001 || backbone_AvrII_fw || ttttcctaggtagcggcgcattaagcgcgg | + | |align="right"| D001 || backbone_AvrII_fw || ttttcctaggtagcggcgcattaagcgcgg |
|- | |- | ||
- | |align="right"| D002 || backbone_AvrII_rev || ttttcctaggcttgttgtagcttaaattttg | + | |align="right"| D002 || backbone_AvrII_rev || ttttcctaggcttgttgtagcttaaattttg |
|- | |- | ||
- | |align="right"| D003 || backbone_ClaI_fw || ttttatcgattagcggcgcattaagcgcgg | + | |align="right"| D003 || backbone_ClaI_fw || ttttatcgattagcggcgcattaagcgcgg |
|- | |- | ||
- | |align="right"| D004 || backbone_XhoI_rev || ttttctcgagcttgttgtagcttaaattttg | + | |align="right"| D004 || backbone_XhoI_rev || ttttctcgagcttgttgtagcttaaattttg |
|- | |- | ||
- | |align="right"| D005 || BBB_Oligo_XhoI || ccggActgcagcggccgcgcgctagcacactagtgcggccgcgaattCa | + | |align="right"| D005 || BBB_Oligo_XhoI || ccggActgcagcggccgcgcgctagcacactagtgcggccgcgaattCa |
|- | |- | ||
- | |align="right"| D006 || BBB_Oligo_XmaI || tcgatgaattcgcggccgcactagtgtgctagcgcgcggccgctgcagt | + | |align="right"| D006 || BBB_Oligo_XmaI || tcgatgaattcgcggccgcactagtgtgctagcgcgcggccgctgcagt |
|- | |- | ||
- | |align="right"| D007 || BBB_Oligo_XmaI || tcgatgaattcgcggccgcactagtgtgctagcgcgcggccgctgcagt | + | |align="right"| D007 || BBB_Oligo_XmaI || tcgatgaattcgcggccgcactagtgtgctagcgcgcggccgctgcagt |
|- | |- | ||
- | |align="right"| D008 || BBB_suffix_reverse || aaaactgcagcggccgcgc | + | |align="right"| D008 || BBB_suffix_reverse || aaaactgcagcggccgcgc |
|- | |- | ||
- | |align="right"| D009 || BGH_pA_BamHI_rc_fw || ttttgaattcgcggccgcactagtggatccccatagagcccaccgcatccc | + | |align="right"| D009 || BGH_pA_BamHI_rc_fw || ttttgaattcgcggccgcactagtggatccccatagagcccaccgcatccc |
|- | |- | ||
- | |align="right"| D010 || BGH_pA_fw || ttttgaattcgcggccgcactagttttaaacccgctgatcagcc | + | |align="right"| D010 || BGH_pA_fw || ttttgaattcgcggccgcactagttttaaacccgctgatcagcc |
|- | |- | ||
- | |align="right"| D011 || BGH_pA_rev || ttttctgcagcggccgcgctagcccatagagcccaccgcatccc | + | |align="right"| D011 || BGH_pA_rev || ttttctgcagcggccgcgctagcccatagagcccaccgcatccc |
|- | |- | ||
- | |align="right"| D012 || BGH_pA_rc_rev || ttttctgcagcggccgcgctagctttaaacccgctgatcagcc | + | |align="right"| D012 || BGH_pA_rc_rev || ttttctgcagcggccgcgctagctttaaacccgctgatcagcc |
|- | |- | ||
- | |align="right"| D013 || biCMV_BBB_fw || ttttgaattcgcggccgcactagtgcgatctgacggttcactaaacg | + | |align="right"| D013 || biCMV_BBB_fw || ttttgaattcgcggccgcactagtgcgatctgacggttcactaaacg |
|- | |- | ||
- | |align="right"| D014 || biCMV_BBB_fw || ttttgaattcgcggccgcactagtgcgatctgacggttcactaaacg | + | |align="right"| D014 || biCMV_BBB_fw || ttttgaattcgcggccgcactagtgcgatctgacggttcactaaacg |
|- | |- | ||
- | |align="right"| D015 || biCMV_BBB_rev || ttttctgcagcggccgcgcgctagcacagcggatctgacggttcac | + | |align="right"| D015 || biCMV_BBB_rev || ttttctgcagcggccgcgcgctagcacagcggatctgacggttcac |
|- | |- | ||
- | |align="right"| D016 || biCMV_rc_BBB_fw || ttttgaattcgcggccgcactagtagcggatctgacggttcac | + | |align="right"| D016 || biCMV_rc_BBB_fw || ttttgaattcgcggccgcactagtagcggatctgacggttcac |
|- | |- | ||
- | |align="right"| D017 || biCMV_rc_BBB_rev || ttttctgcagcggccgcgctagcgcgatctgacggttcac | + | |align="right"| D017 || biCMV_rc_BBB_rev || ttttctgcagcggccgcgctagcgcgatctgacggttcac |
|- | |- | ||
- | |align="right"| D018 || CMV_BBB_BamHI_fw || gaattcgcggccgcactagtggatcccgatgtacgggccagatatacg | + | |align="right"| D018 || CMV_BBB_BamHI_fw || gaattcgcggccgcactagtggatcccgatgtacgggccagatatacg |
|- | |- | ||
- | |align="right"| D019 || CMV_BBB_fw || ttttgaattcgcggccgcactagtcgatgtacgggccagatatacg | + | |align="right"| D019 || CMV_BBB_fw || ttttgaattcgcggccgcactagtcgatgtacgggccagatatacg |
|- | |- | ||
- | |align="right"| D020 || CMV_BBB_rev || ttttctgcagcggccgcgcgctagcacatttcgataagccagtaagc | + | |align="right"| D020 || CMV_BBB_rev || ttttctgcagcggccgcgcgctagcacatttcgataagccagtaagc |
|- | |- | ||
- | |align="right"| D021 || CMV_Teto2_BBB_fw || tttt gaattcgcggccgcactagtgttgacattgattattgtctag | + | |align="right"| D021 || CMV_Teto2_BBB_fw || tttt gaattcgcggccgcactagtgttgacattgattattgtctag |
|- | |- | ||
- | |align="right"| D022 || CMV_TetO2_BBB_rev || tttt ctgcagcggccgcgcgctagcaccggaggctggatcggtc | + | |align="right"| D022 || CMV_TetO2_BBB_rev || tttt ctgcagcggccgcgcgctagcaccggaggctggatcggtc |
|- | |- | ||
- | |align="right"| D023 || CMV_rc_BBB_fw || ttttgaattcgcggccgcactagtatttcgataagccagtaagc | + | |align="right"| D023 || CMV_rc_BBB_fw || ttttgaattcgcggccgcactagtatttcgataagccagtaagc |
|- | |- | ||
- | |align="right"| D024 || CMV_rc_BBB_rev || ttttctgcagcggccgcgctagccgatgtacgggccagatatacg | + | |align="right"| D024 || CMV_rc_BBB_rev || ttttctgcagcggccgcgctagccgatgtacgggccagatatacg |
|- | |- | ||
- | |align="right"| D025 || FRT_rev || ctgcagcggccgcgctagcccaaggaagttcctatactttc | + | |align="right"| D025 || FRT_rev || ctgcagcggccgcgctagcccaaggaagttcctatactttc |
|- | |- | ||
- | |align="right"| D026 || FRT_rev || gaattcgcggccgcactagtttctcgccacgttcgccggc | + | |align="right"| D026 || FRT_rev || gaattcgcggccgcactagtttctcgccacgttcgccggc |
|- | |- | ||
- | |align="right"| D027 || hRluc_ter_BBB_fw || ttttgaattcgcggccgcactagtatggcttccaaggtgtacgac | + | |align="right"| D027 || hRluc_ter_BBB_fw || ttttgaattcgcggccgcactagtatggcttccaaggtgtacgac |
|- | |- | ||
- | |align="right"| D028 || hRluc_ter_BBB_rev || ttttctgcagcggccgcgctagcttactgctcgttcttcagcacgc | + | |align="right"| D028 || hRluc_ter_BBB_rev || ttttctgcagcggccgcgctagcttactgctcgttcttcagcacgc |
|- | |- | ||
- | |align="right"| D029 || hsa-mir-122_BBB(perf) || ttttgaattcgcggccgcactagtcaaacaccattgtcacactccagctagcgcggccgctgcagtttt | + | |align="right"| D029 || hsa-mir-122_BBB(perf) || ttttgaattcgcggccgcactagtcaaacaccattgtcacactccagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D030 || hsa-mir-122_BBB(rand9-12) || ttttgaattcgcggccgcactagtcaaacaccatnnnnacactccagctagcgcggccgctgcagtttt | + | |align="right"| D030 || hsa-mir-122_BBB(rand9-12) || ttttgaattcgcggccgcactagtcaaacaccatnnnnacactccagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D031 || hsa-mir-122_BBB(rand9-22) || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnnacactccagctagcgcggccgctgcagtttt | + | |align="right"| D031 || hsa-mir-122_BBB(rand9-22) || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnnacactccagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D032 || hsa-mir-122_Sgf_Not(perf) || ttttgcgatcgccaaacaccattgtcacactccagcggccgctatgtcacaact | + | |align="right"| D032 || hsa-mir-122_Sgf_Not(perf) || ttttgcgatcgccaaacaccattgtcacactccagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D033 || hsa-mir-122_Sgf_Not(rand9-12) || ttttgcgatcgccaaacaccatnnnnacactccagcggccgctatgtcacaact | + | |align="right"| D033 || hsa-mir-122_Sgf_Not(rand9-12) || ttttgcgatcgccaaacaccatnnnnacactccagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D034 || hsa-mir-122_Sgf_Not(rand9-22) || ttttgcgatcgcnnnnnnnnnnnnnnacactccagcggccgctatgtcacaact | + | |align="right"| D034 || hsa-mir-122_Sgf_Not(rand9-22) || ttttgcgatcgcnnnnnnnnnnnnnnacactccagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D035 || hsa-mir-375_BBB(perf) || ttttgaattcgcggccgcactagttcacgcgagccgaacgaacaaagctagcgcggccgctgcagtttt | + | |align="right"| D035 || hsa-mir-375_BBB(perf) || ttttgaattcgcggccgcactagttcacgcgagccgaacgaacaaagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D036 || hsa-mir-375_BBB(rand9-12) || ttttgaattcgcggccgcactagttcacgcgagcnnnncgaacaaagctagcgcggccgctgcagtttt | + | |align="right"| D036 || hsa-mir-375_BBB(rand9-12) || ttttgaattcgcggccgcactagttcacgcgagcnnnncgaacaaagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D037 || hsa-mir-375_BBB(rand9-22) || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnncgaacaaagctagcgcggccgctgcagtttt | + | |align="right"| D037 || hsa-mir-375_BBB(rand9-22) || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnncgaacaaagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D038 || hsa-mir-375_Sgf_Not(perf) || ttttgcgatcgctcacgcgagccgaacgaacaaagcggccgctatgtcacaact | + | |align="right"| D038 || hsa-mir-375_Sgf_Not(perf) || ttttgcgatcgctcacgcgagccgaacgaacaaagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D039 || hsa-mir-375_Sgf_Not(rand9-12) || ttttgcgatcgctcacgcgagcnnnncgaacaaagcggccgctatgtcacaact | + | |align="right"| D039 || hsa-mir-375_Sgf_Not(rand9-12) || ttttgcgatcgctcacgcgagcnnnncgaacaaagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D040 || hsa-mir-375_Sgf_Not(rand9-22) || ttttgcgatcgcnnnnnnnnnnnnnncgaacaaagcggccgctatgtcacaact | + | |align="right"| D040 || hsa-mir-375_Sgf_Not(rand9-22) || ttttgcgatcgcnnnnnnnnnnnnnncgaacaaagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D041 || hsa-mir-376a_BBB(perf) || ttttgaattcgcggccgcactagtacgtggattttcctctatgatgctagcgcggccgctgcagtttt | + | |align="right"| D041 || hsa-mir-376a_BBB(perf) || ttttgaattcgcggccgcactagtacgtggattttcctctatgatgctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D042 || hsa-mir-376a_BBB(rand9-12) || ttttgaattcgcggccgcactagtacgtggattnnnntctatgatgctagcgcggccgctgcagtttt | + | |align="right"| D042 || hsa-mir-376a_BBB(rand9-12) || ttttgaattcgcggccgcactagtacgtggattnnnntctatgatgctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D043 || hsa-mir-376a_BBB(rand9-22) || ttttgaattcgcggccgcactagtnnnnnnnnnnnnntctatgatgctagcgcggccgctgcagtttt | + | |align="right"| D043 || hsa-mir-376a_BBB(rand9-22) || ttttgaattcgcggccgcactagtnnnnnnnnnnnnntctatgatgctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D044 || hsa-mir-376a_Sgf_Not(perf) || ttttgcgatcgcacgtggattttcctctatgatgcggccgctatgtcacaact | + | |align="right"| D044 || hsa-mir-376a_Sgf_Not(perf) || ttttgcgatcgcacgtggattttcctctatgatgcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D045 || hsa-mir-376a_Sgf_Not(rand9-12) || ttttgcgatcgcacgtggattnnnntctatgatgcggccgctatgtcacaact | + | |align="right"| D045 || hsa-mir-376a_Sgf_Not(rand9-12) || ttttgcgatcgcacgtggattnnnntctatgatgcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D046 || hsa-mir-376a_Sgf_Not(rand9-22) || ttttgcgatcgcnnnnnnnnnnnnntctatgatgcggccgctatgtcacaact | + | |align="right"| D046 || hsa-mir-376a_Sgf_Not(rand9-22) || ttttgcgatcgcnnnnnnnnnnnnntctatgatgcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D047 || igem20100723_01 || tgccacctgacgtctaagaa | + | |align="right"| D047 || igem20100723_01 || tgccacctgacgtctaagaa |
|- | |- | ||
- | |align="right"| D048 || igem20100723_02 || attaccgcctttgagtgagc | + | |align="right"| D048 || igem20100723_02 || attaccgcctttgagtgagc |
|- | |- | ||
- | |align="right"| D049 || Luc2_ter_BamHI_BBB_rev || ttttctgcagcggccgcgctagcggatcctaccacatttgtagaggttttac | + | |align="right"| D049 || Luc2_ter_BamHI_BBB_rev || ttttctgcagcggccgcgctagcggatcctaccacatttgtagaggttttac |
|- | |- | ||
- | |align="right"| D050 || Luc2_ter_BamHI_BBB_rev || ttttctgcagcggccgcgctagcggatcctaccacatttgtagaggttttac | + | |align="right"| D050 || Luc2_ter_BamHI_BBB_rev || ttttctgcagcggccgcgctagcggatcctaccacatttgtagaggttttac |
|- | |- | ||
- | |align="right"| D051 || Luc2_ter_BBB_fw || ttttgaattcgcggccgcactagtgccaccatggaagatgcc | + | |align="right"| D051 || Luc2_ter_BBB_fw || ttttgaattcgcggccgcactagtgccaccatggaagatgcc |
|- | |- | ||
- | |align="right"| D052 || mir122_miMeasure_screen_fev || ccgcgctagctggagtgt | + | |align="right"| D052 || mir122_miMeasure_screen_fev || ccgcgctagctggagtgt |
|- | |- | ||
- | |align="right"| D053 || Oligo_Xba_mut_fw || ctagcctcgagttctgaccgccccgggg | + | |align="right"| D053 || Oligo_Xba_mut_fw || ctagcctcgagttctgaccgccccgggg |
|- | |- | ||
- | |align="right"| D054 || Oligo_Xba_mut_rev || ctagccccggggcggtcagaactcgagg | + | |align="right"| D054 || Oligo_Xba_mut_rev || ctagccccggggcggtcagaactcgagg |
|- | |- | ||
- | |align="right"| D055 || pSMB_miMeasure_Screen_fw || gcacaagctggagtacaactac | + | |align="right"| D055 || pSMB_miMeasure_Screen_fw || gcacaagctggagtacaactac |
|- | |- | ||
- | |align="right"| D056 || pSMB_miMeasure_Screen_rev || ggtttcccgactggaaagcg | + | |align="right"| D056 || pSMB_miMeasure_Screen_rev || ggtttcccgactggaaagcg |
|- | |- | ||
- | |align="right"| D057 || RSV_fw || ttttgaattcgcggccgcactagtcaattctcatgtttgacagc | + | |align="right"| D057 || RSV_fw || ttttgaattcgcggccgcactagtcaattctcatgtttgacagc |
|- | |- | ||
- | |align="right"| D058 || RSV_rev || ttttctgcagcggccgcgctagccagcttggaggtgcacacc | + | |align="right"| D058 || RSV_rev || ttttctgcagcggccgcgctagccagcttggaggtgcacacc |
|- | |- | ||
- | |align="right"| D059 || RSV_rc_fw || ttttgaattcgcggccgcactagtcagcttggaggtgcacacc | + | |align="right"| D059 || RSV_rc_fw || ttttgaattcgcggccgcactagtcagcttggaggtgcacacc |
|- | |- | ||
- | |align="right"| D060 || RSV_rc_rev || ttttctgcagcggccgcgctagccaattctcatgtttgacagc | + | |align="right"| D060 || RSV_rc_rev || ttttctgcagcggccgcgctagccaattctcatgtttgacagc |
|- | |- | ||
- | |align="right"| D061 || RSV_fw_midcomp || cgaaccactgaataccgcattgcag | + | |align="right"| D061 || RSV_fw_midcomp || cgaaccactgaataccgcattgcag |
|- | |- | ||
- | |align="right"| D062 || RSV_rev_midcomp || ctgcaatgcggtattcagtggttcg | + | |align="right"| D062 || RSV_rev_midcomp || ctgcaatgcggtattcagtggttcg |
|- | |- | ||
- | |align="right"| D063 || SB-prep-3P || gccgctgcagtccggcaaaaaaacg | + | |align="right"| D063 || SB-prep-3P || gccgctgcagtccggcaaaaaaacg |
|- | |- | ||
- | |align="right"| D064 || SB-prep-2Ea || atgaattccagaaatcatccttagcg | + | |align="right"| D064 || SB-prep-2Ea || atgaattccagaaatcatccttagcg |
|- | |- | ||
- | |align="right"| D065 || second_strand_notI || agttgtgacatagcggccgc | + | |align="right"| D065 || second_strand_notI || agttgtgacatagcggccgc |
|- | |- | ||
- | |align="right"| D066 || shRNA-6th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnncgtcaatagctagcgcggccgctgcagtttt | + | |align="right"| D066 || shRNA-6th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnncgtcaatagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D067 || shRNA-7th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnntcaatagagctagcgcggccgctgcagtttt | + | |align="right"| D067 || shRNA-7th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnntcaatagagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D068 || shRNA-8th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnngctgatgtgctagcgcggccgctgcagtttt | + | |align="right"| D068 || shRNA-8th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnngctgatgtgctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D069 || shRNA-9th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnnctagtttagctagcgcggccgctgcagtttt | + | |align="right"| D069 || shRNA-9th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnnctagtttagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D070 || shRNA-10th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnngctataatgctagcgcggccgctgcagtttt | + | |align="right"| D070 || shRNA-10th-bs(custom)_BBB || ttttgaattcgcggccgcactagtnnnnnnnnnnnnnngctataatgctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D071 || shRNA-6th-bs(perf)_BBB || ttttgaattcgcggccgcactagtcgcaacctcactgccgtcaatagctagcgcggccgctgcagtttt | + | |align="right"| D071 || shRNA-6th-bs(perf)_BBB || ttttgaattcgcggccgcactagtcgcaacctcactgccgtcaatagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D072 || shRNA-7th-bs(perf)_BBB || ttttgaattcgcggccgcactagtcaacctcactgccgtcaatagagctagcgcggccgctgcagtttt | + | |align="right"| D072 || shRNA-7th-bs(perf)_BBB || ttttgaattcgcggccgcactagtcaacctcactgccgtcaatagagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D073 || shRNA-8th-bs(perf)_BBB || ttttgaattcgcggccgcactagtcaacgacgtatttggctgatgtgctagcgcggccgctgcagtttt | + | |align="right"| D073 || shRNA-8th-bs(perf)_BBB || ttttgaattcgcggccgcactagtcaacgacgtatttggctgatgtgctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D074 || shRNA-9th-bs(perf)_BBB || ttttgaattcgcggccgcactagtcgcgacatagcgacctagtttagctagcgcggccgctgcagtttt | + | |align="right"| D074 || shRNA-9th-bs(perf)_BBB || ttttgaattcgcggccgcactagtcgcgacatagcgacctagtttagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D075 || shRNA-10th-bs(perf)_BBB || ttttgaattcgcggccgcactagtctgtggtgccggtggctataatgctagcgcggccgctgcagtttt | + | |align="right"| D075 || shRNA-10th-bs(perf)_BBB || ttttgaattcgcggccgcactagtctgtggtgccggtggctataatgctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D076 || shRNA-6th-bs(rand9-12)_BBB || gcgatcgccgcaacctcannnncgtcaatagcggccgctatgtcacaact | + | |align="right"| D076 || shRNA-6th-bs(rand9-12)_BBB || gcgatcgccgcaacctcannnncgtcaatagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D077 || shRNA-7th-bs(rand9-12)_BBB || ttttgaattcgcggccgcactagtcaacctcactnnnntcaatagagctagcgcggccgctgcagtttt | + | |align="right"| D077 || shRNA-7th-bs(rand9-12)_BBB || ttttgaattcgcggccgcactagtcaacctcactnnnntcaatagagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D078 || shRNA-8th-bs(rand9-12)_BBB || ttttgaattcgcggccgcactagtcaacgacgtannnngctgatgtgctagcgcggccgctgcagtttt | + | |align="right"| D078 || shRNA-8th-bs(rand9-12)_BBB || ttttgaattcgcggccgcactagtcaacgacgtannnngctgatgtgctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D079 || shRNA-9th-bs(rand9-12)_BBB || ttttgaattcgcggccgcactagtcgcgacatagnnnnctagtttagctagcgcggccgctgcagtttt | + | |align="right"| D079 || shRNA-9th-bs(rand9-12)_BBB || ttttgaattcgcggccgcactagtcgcgacatagnnnnctagtttagctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D080 || shRNA-10th-bs(rand9-12)_BBB || ttttgaattcgcggccgcactagtctgtggtgccnnnngctataatgctagcgcggccgctgcagtttt | + | |align="right"| D080 || shRNA-10th-bs(rand9-12)_BBB || ttttgaattcgcggccgcactagtctgtggtgccnnnngctataatgctagcgcggccgctgcagtttt |
|- | |- | ||
- | |align="right"| D081 || shRNA-6th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnncgtcaatagcggccgctatgtcacaact | + | |align="right"| D081 || shRNA-6th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnncgtcaatagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D082 || shRNA-7th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnntcaatagagcggccgctatgtcacaact | + | |align="right"| D082 || shRNA-7th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnntcaatagagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D083 || shRNA-8th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnngctgatgtgcggccgctatgtcacaact | + | |align="right"| D083 || shRNA-8th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnngctgatgtgcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D084 || shRNA-9th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnnctagtttagcggccgctatgtcacaact | + | |align="right"| D084 || shRNA-9th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnnctagtttagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D085 || shRNA-10th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnngctataatgcggccgctatgtcacaact | + | |align="right"| D085 || shRNA-10th-bs(custom)_SgfI_NotI || gcgatcgcnnnnnnnnnnnnnngctataatgcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D086 || shRNA-6th-bs(perf)_SgfI_NotI || gcgatcgccgcaacctcactgccgtcaatagcggccgctatgtcacaact | + | |align="right"| D086 || shRNA-6th-bs(perf)_SgfI_NotI || gcgatcgccgcaacctcactgccgtcaatagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D087 || shRNA-7th-bs(perf)_SgfI_NotI || gcgatcgccaacctcactgccgtcaatagagcggccgctatgtcacaact | + | |align="right"| D087 || shRNA-7th-bs(perf)_SgfI_NotI || gcgatcgccaacctcactgccgtcaatagagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D088 || shRNA-8th-bs(perf)_SgfI_NotI || gcgatcgccaacgacgtatttggctgatgtgcggccgctatgtcacaact | + | |align="right"| D088 || shRNA-8th-bs(perf)_SgfI_NotI || gcgatcgccaacgacgtatttggctgatgtgcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D089 || shRNA-9th-bs(perf)_SgfI_NotI || gcgatcgccgcgacatagcgacctagtttagcggccgctatgtcacaact | + | |align="right"| D089 || shRNA-9th-bs(perf)_SgfI_NotI || gcgatcgccgcgacatagcgacctagtttagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D090 || shRNA-10th-bs(perf)_SgfI_NotI || gcgatcgcctgtggtgccggtggctataatgcggccgctatgtcacaact | + | |align="right"| D090 || shRNA-10th-bs(perf)_SgfI_NotI || gcgatcgcctgtggtgccggtggctataatgcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D091 || shRNA-6th-bs(rand9-12)_SgfI_NotI || gcgatcgccgcaacctcannnncgtcaatagcggccgctatgtcacaact | + | |align="right"| D091 || shRNA-6th-bs(rand9-12)_SgfI_NotI || gcgatcgccgcaacctcannnncgtcaatagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D092 || shRNA-7th-bs(rand9-12)_SgfI_NotI || gcgatcgccaacctcactnnnntcaatagagcggccgctatgtcacaact | + | |align="right"| D092 || shRNA-7th-bs(rand9-12)_SgfI_NotI || gcgatcgccaacctcactnnnntcaatagagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D093 || shRNA-8th-bs(rand9-12)_SgfI_NotI || gcgatcgccaacgacgtannnngctgatgtgcggccgctatgtcacaact | + | |align="right"| D093 || shRNA-8th-bs(rand9-12)_SgfI_NotI || gcgatcgccaacgacgtannnngctgatgtgcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D094 || shRNA-9th-bs(rand9-12)_SgfI_NotI || gcgatcgccgcgacatagnnnnctagtttagcggccgctatgtcacaact | + | |align="right"| D094 || shRNA-9th-bs(rand9-12)_SgfI_NotI || gcgatcgccgcgacatagnnnnctagtttagcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D095 || shRNA-10th-bs(rand9-12)_SgfI_NotI || gcgatcgcctgtggtgccnnnngctataatgcggccgctatgtcacaact | + | |align="right"| D095 || shRNA-10th-bs(rand9-12)_SgfI_NotI || gcgatcgcctgtggtgccnnnngctataatgcggccgctatgtcacaact |
|- | |- | ||
- | |align="right"| D096 || shRNA_AflII_BBB_fw || ttttctgcagcggccgcgcgctagccttaagtggaggtgaagttaacaccttcgtg | + | |align="right"| D096 || shRNA_AflII_BBB_fw || ttttctgcagcggccgcgcgctagccttaagtggaggtgaagttaacaccttcgtg |
|- | |- | ||
- | |align="right"| D097 || shRNA_HindIII_BBB_rev || ttttgaattcgcggccgcactagtaagcttaagcaaacgatgccaagacatttatcg | + | |align="right"| D097 || shRNA_HindIII_BBB_rev || ttttgaattcgcggccgcactagtaagcttaagcaaacgatgccaagacatttatcg |
|- | |- | ||
- | |align="right"| D098 || SV40_BamHI_BBB_fw || ctgcagcggccgcgctagcggatccaagctttttgcaaaagcctagg | + | |align="right"| D098 || SV40_BamHI_BBB_fw || ctgcagcggccgcgctagcggatccaagctttttgcaaaagcctagg |
|- | |- | ||
- | |align="right"| D099 || SV40_BBB_rev || ttttctgcagcggccgcgctagcaagctttttgcaaaagcctaggc | + | |align="right"| D099 || SV40_BBB_rev || ttttctgcagcggccgcgctagcaagctttttgcaaaagcctaggc |
|- | |- | ||
- | |align="right"| D100 || SV40_rc_BBB_fw || ttttgaattcgcggccgcactagtaagctttttgcaaaagcc | + | |align="right"| D100 || SV40_rc_BBB_fw || ttttgaattcgcggccgcactagtaagctttttgcaaaagcc |
|- | |- | ||
- | |align="right"| D101 || SV40_rc_BBB_rev || ttttctgcagcggccgcgctagcgcgcagcaccatggcctg | + | |align="right"| D101 || SV40_rc_BBB_rev || ttttctgcagcggccgcgctagcgcgcagcaccatggcctg |
|- | |- | ||
- | |align="right"| D102 || SV40_term_fw || ttttgaattcgcggccgcactagtcagacatgataagatacattg | + | |align="right"| D102 || SV40_term_fw || ttttgaattcgcggccgcactagtcagacatgataagatacattg |
|- | |- | ||
- | |align="right"| D103 || SV40_Xho_Xma_BBB_fw || gaattcgcggccgcactagtctcgagtttctcccggggcgcagcaccatggcctg | + | |align="right"| D103 || SV40_Xho_Xma_BBB_fw || gaattcgcggccgcactagtctcgagtttctcccggggcgcagcaccatggcctg |
|- | |- | ||
- | |align="right"| D104 || TetRepressor (from pcDNA6)fw || ttttgaattcgcggccgcactagtgccaccatgtctagattagataaaag | + | |align="right"| D104 || TetRepressor (from pcDNA6)fw || ttttgaattcgcggccgcactagtgccaccatgtctagattagataaaag |
|- | |- | ||
- | |align="right"| D105 || TetRepressor (from pcDNA6)rew || ttttctgcagcggccgcgctagcttaataagatctaaattcccgcgatccgc | + | |align="right"| D105 || TetRepressor (from pcDNA6)rew || ttttctgcagcggccgcgctagcttaataagatctaaattcccgcgatccgc |
|} | |} | ||
- | |||
- | |||
==ViroBytes primers== | ==ViroBytes primers== | ||
- | |||
{| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | {| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | ||
!align="right"| ID !! Name !! Sequence (5' to 3') !! amplified fragment !! direction !! cap gene of AAV | !align="right"| ID !! Name !! Sequence (5' to 3') !! amplified fragment !! direction !! cap gene of AAV | ||
|- | |- | ||
- | |align="right"| R001 || | + | |align="right"| R001 || Anchor12689 || tctctctctctctctaagcttgtctgagtgactagcattcgttaattaacTACCG || anchor || - || |
|- | |- | ||
|align="right"| R002 || Anchor4 || tctctctctctctctaagcttgtctgagtgactagcattcgttaattaactactg || anchor || - || | |align="right"| R002 || Anchor4 || tctctctctctctctaagcttgtctgagtgactagcattcgttaattaactactg || anchor || - || | ||
Line 344: | Line 340: | ||
|align="right"| R004 || Anchor_comp_all || gttaattaacgaatgctagtcactcagacaagctt || anchor || - || | |align="right"| R004 || Anchor_comp_all || gttaattaacgaatgctagtcactcagacaagctt || anchor || - || | ||
|- | |- | ||
- | |align="right"| R005 || | + | |align="right"| R005 || F1_for_cap12689 || gctacggtctcaatggctgccgatggttatcttccag || 1 || forward || 1,2,6,8,9 |
|- | |- | ||
|align="right"| R006 || F1_for_cap5 || gctacggtctcaatggctgccgatggttatcttccag || 1 || forward || 5 | |align="right"| R006 || F1_for_cap5 || gctacggtctcaatggctgccgatggttatcttccag || 1 || forward || 5 | ||
Line 500: | Line 496: | ||
|align="right"| R082 || F8_rev_cap9 || gctacggcgcgccttacagattacgagtcaggtatctggtgccaatggg || 8 || reverse || 9 | |align="right"| R082 || F8_rev_cap9 || gctacggcgcgccttacagattacgagtcaggtatctggtgccaatggg || 8 || reverse || 9 | ||
|- | |- | ||
- | |align="right"| R083 || Virbyte_for || | + | |align="right"| R083 || Virbyte_for || gtctgagtgactagcattcg || - || forward || |
+ | |- | ||
+ | |align="right"| R084 || Virobyte_rev || gctacggcgcgcctta || - || reverse || | ||
+ | |- | ||
+ | |align="right"| R085 || Anchor F4F5 12689 || || anchor || - || | ||
+ | |- | ||
+ | |align="right"| R086 || Anchor F4F5 5 || || anchor || - || | ||
+ | |- | ||
+ | |align="right"| R087 || Anchor_F4F5 comp_all || || anchor || - || | ||
|- | |- | ||
- | |||
|} | |} | ||
- | |||
- | |||
==Primer Table Quick 'n' Dirty== | ==Primer Table Quick 'n' Dirty== | ||
- | |||
{| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | {| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | ||
Line 535: | Line 535: | ||
|align="right"| L011 || TetR_SgfI_NotI_rv || ttttgcggccgctggcgatcgctaataagatctgaattcccgggatc || for use with L12 || from pcDNA6/TR || to pTR-UF3 BspEI/NotI | |align="right"| L011 || TetR_SgfI_NotI_rv || ttttgcggccgctggcgatcgctaataagatctgaattcccgggatc || for use with L12 || from pcDNA6/TR || to pTR-UF3 BspEI/NotI | ||
|} | |} | ||
- | |||
==Primer Table raPCR == | ==Primer Table raPCR == | ||
- | |||
{| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | {| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | ||
Line 586: | Line 584: | ||
- | + | ==mouse oligos== | |
- | + | ||
- | == | + | |
- | + | ||
{| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | {| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | ||
- | !align="right"| ID !! Name !! Sequence (5' to 3') | + | !align="right"| ID !! Name !! Sequence (5' to 3') |
|- | |- | ||
- | |align="right"| mouse1 || CMV_TetR_122bs_SalI_fw ||ttttgtcgaccgttgacattgattattgac | + | |align="right"| mouse1 || CMV_TetR_122bs_SalI_fw ||ttttgtcgaccgttgacattgattattgac |
|- | |- | ||
- | |align="right"| mouae2 || CMV_TetR_both_MluI_rev || ttttacgcgttaacacacaaaaaaccaacac | + | |align="right"| mouae2 || CMV_TetR_both_MluI_rev || ttttacgcgttaacacacaaaaaaccaacac |
|- | |- | ||
- | |align="right"| mouse3 || CMV_TetR_control_SalI_fw || ttttgtcgaccgttgacattgattattgac | + | |align="right"| mouse3 || CMV_TetR_control_SalI_fw || ttttgtcgaccgttgacattgattattgac |
|- | |- | ||
- | |align="right"| mouse4 || CMV_TetO2_EcoRI_fw || ttttgaattcgttgacattgattattgtctag | + | |align="right"| mouse4 || CMV_TetO2_EcoRI_fw || ttttgaattcgttgacattgattattgtctag |
|- | |- | ||
- | |align="right"| mouse5 || Luc2_Ter_PstI_rev || ttttctgcagtaccacatttgtagaggttttac | + | |align="right"| mouse5 || Luc2_Ter_PstI_rev || ttttctgcagtaccacatttgtagaggttttac |
|- | |- | ||
- | |align="right"| | + | |align="right"| mouse6XXX || haat_bs_p_fw || tcgaggaagcgtttaggcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse7 || haat_bs_p_rev || ggccgctctagagatgttaaacatgcctaaacgcttcc | + | |align="right"| mouse7 || haat_bs_p_rev || ggccgctctagagatgttaaacatgcctaaacgcttcc |
|- | |- | ||
- | |align="right"| mouse8 || haat_bs_r10_12_aat_fw || tcgaggaagcgtaatggcatgtttaacatctctagagc | + | |align="right"| mouse8 || haat_bs_r10_12_aat_fw || tcgaggaagcgtaatggcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse9 || haat_bs_r10_12_aat_rev || ggccgctctagagatgttaaacatgccattacgcttcc | + | |align="right"| mouse9 || haat_bs_r10_12_aat_rev || ggccgctctagagatgttaaacatgccattacgcttcc |
|- | |- | ||
- | |align="right"| mouse10 || haat_bs_r10_12_agc_fw || tcgaggaagcgtagcggcatgtttaacatctctagagc | + | |align="right"| mouse10 || haat_bs_r10_12_agc_fw || tcgaggaagcgtagcggcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse11 || haat_bs_r10_12_agc_rev || ggccgctctagagatgttaaacatgccgctacgcttcc | + | |align="right"| mouse11 || haat_bs_r10_12_agc_rev || ggccgctctagagatgttaaacatgccgctacgcttcc |
|- | |- | ||
- | |align="right"| mouse12 || haat_bs_m10at_fw || tcgaggaagcgttttggcatgtttaacatctctagagc | + | |align="right"| mouse12 || haat_bs_m10at_fw || tcgaggaagcgttttggcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse13 || haat_bs_m10at_rev || ggccgctctagagatgttaaacatgccaaaacgcttcc | + | |align="right"| mouse13 || haat_bs_m10at_rev || ggccgctctagagatgttaaacatgccaaaacgcttcc |
|- | |- | ||
- | |align="right"| mouse14 || haat_bs_m10ac_fw || tcgaggaagcgtttcggcatgtttaacatctctagagc | + | |align="right"| mouse14 || haat_bs_m10ac_fw || tcgaggaagcgtttcggcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse15 || haat_bs_m10ac_rev || ggccgctctagagatgttaaacatgccgaaacgcttcc | + | |align="right"| mouse15 || haat_bs_m10ac_rev || ggccgctctagagatgttaaacatgccgaaacgcttcc |
|- | |- | ||
- | |align="right"| mouse16 || haat_bs_m11ta_fw || tcgaggaagcgttaaggcatgtttaacatctctagagc | + | |align="right"| mouse16 || haat_bs_m11ta_fw || tcgaggaagcgttaaggcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse17 || haat_bs_m11ta_rev || ggccgctctagagatgttaaacatgccttaacgcttcc | + | |align="right"| mouse17 || haat_bs_m11ta_rev || ggccgctctagagatgttaaacatgccttaacgcttcc |
|- | |- | ||
- | |align="right"| mouse18 || haat_bs_m11tg_fw || tcgaggaagcgttgaggcatgtttaacatctctagagc | + | |align="right"| mouse18 || haat_bs_m11tg_fw || tcgaggaagcgttgaggcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse19 || haat_bs_m11tg_rev || ggccgctctagagatgttaaacatgcctcaacgcttcc | + | |align="right"| mouse19 || haat_bs_m11tg_rev || ggccgctctagagatgttaaacatgcctcaacgcttcc |
|- | |- | ||
- | |align="right"| mouse20 || haat_bs_r9_12_aatc_fw || tcgaggaagcgtaatcgcatgtttaacatctctagagc | + | |align="right"| mouse20 || haat_bs_r9_12_aatc_fw || tcgaggaagcgtaatcgcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse21 || haat_bs_r9_12_aatc_rev || ggccgctctagagatgttaaacatgcgattacgcttcc | + | |align="right"| mouse21 || haat_bs_r9_12_aatc_rev || ggccgctctagagatgttaaacatgcgattacgcttcc |
|- | |- | ||
- | |align="right"| mouse22 || haat_bs_r9_12_agcc_fw || tcgaggaagcgtagccgcatgtttaacatctctagagc | + | |align="right"| mouse22 || haat_bs_r9_12_agcc_fw || tcgaggaagcgtagccgcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse23 || haat_bs_r9_12_agcc_rev || ggccgctctagagatgttaaacatgcggctacgcttcc | + | |align="right"| mouse23 || haat_bs_r9_12_agcc_rev || ggccgctctagagatgttaaacatgcggctacgcttcc |
|- | |- | ||
- | |align="right"| mouse24 || haat_bs_onlyseed_fw || tcgagcttcgcaaatcgcatgtttaacatctctagagc | + | |align="right"| mouse24 || haat_bs_onlyseed_fw || tcgagcttcgcaaatcgcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse25 || haat_bs_onlyseed_rev || ggccgctctagagatgttaaacatgcgatttgcgaagc | + | |align="right"| mouse25 || haat_bs_onlyseed_rev || ggccgctctagagatgttaaacatgcgatttgcgaagc |
|- | |- | ||
- | |align="right"| mouse26 || haat_bs_r16-18_fw || tcgaggttccgtttaggcatgtttaacatctctagagc | + | |align="right"| mouse26 || haat_bs_r16-18_fw || tcgaggttccgtttaggcatgtttaacatctctagagc |
|- | |- | ||
- | |align="right"| mouse27 || haat_bs_r16-18_rev || ggccgctctagagatgttaaacatgcctaaacggaacc | + | |align="right"| mouse27 || haat_bs_r16-18_rev || ggccgctctagagatgttaaacatgcctaaacggaacc |
|- | |- | ||
- | |align="right"| | + | |align="right"| mouse28XXX || sAg_bs_p_fw || tcgagctcagtttactagtgccatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse29 || sAg_bs_p_rev || ggccgctctagagaacaaatggcactagtaaactgagc | + | |align="right"| mouse29 || sAg_bs_p_rev || ggccgctctagagaacaaatggcactagtaaactgagc |
|- | |- | ||
- | |align="right"| mouse30 || sAg_bs_r10_12_acg_fw || tcgagctcagtttactagacgcatttgttctctagagc | + | |align="right"| mouse30 || sAg_bs_r10_12_acg_fw || tcgagctcagtttactagacgcatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse31 || sAg_bs_r10_12_acg_rev || ggccgctctagagaacaaatgcgtctagtaaactgagc | + | |align="right"| mouse31 || sAg_bs_r10_12_acg_rev || ggccgctctagagaacaaatgcgtctagtaaactgagc |
|- | |- | ||
- | |align="right"| mouse32 || sAg_bs_r10_12_taa_fw || tcgagctcagtttactagtaacatttgttctctagagc | + | |align="right"| mouse32 || sAg_bs_r10_12_taa_fw || tcgagctcagtttactagtaacatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse33 || sAg_bs_r10_12_taa_rev || ggccgctctagagaacaaatgttactagtaaactgagc | + | |align="right"| mouse33 || sAg_bs_r10_12_taa_rev || ggccgctctagagaacaaatgttactagtaaactgagc |
|- | |- | ||
- | |align="right"| mouse34 || sAg_bs_r9_12_acgg_fw || tcgagctcagtttactagacggatttgttctctagagc | + | |align="right"| mouse34 || sAg_bs_r9_12_acgg_fw || tcgagctcagtttactagacggatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse35 || sAg_bs_r9_12_acgg_rev || ggccgctctagagaacaaatccgtctagtaaactgagc | + | |align="right"| mouse35 || sAg_bs_r9_12_acgg_rev || ggccgctctagagaacaaatccgtctagtaaactgagc |
|- | |- | ||
- | |align="right"| mouse36 || sAg_bs_r9_12_atgt_fw || tcgagctcagtttactagatgtatttgttctctagagc | + | |align="right"| mouse36 || sAg_bs_r9_12_atgt_fw || tcgagctcagtttactagatgtatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse37 || sAg_bs_r9_12_atgt_rev || ggccgctctagagaacaaatacatctagtaaactgagc | + | |align="right"| mouse37 || sAg_bs_r9_12_atgt_rev || ggccgctctagagaacaaatacatctagtaaactgagc |
|- | |- | ||
- | |align="right"| mouse38 || sAg_bs_m10cg_fw || tcgagctcagtttactagtggcatttgttctctagagc | + | |align="right"| mouse38 || sAg_bs_m10cg_fw || tcgagctcagtttactagtggcatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse39 || sAg_bs_m10cg_rev || ggccgctctagagaacaaatgccactagtaaactgagc | + | |align="right"| mouse39 || sAg_bs_m10cg_rev || ggccgctctagagaacaaatgccactagtaaactgagc |
|- | |- | ||
- | |align="right"| mouse40 || sAg_bs_m10ca_fw || tcgagctcagtttactagtgacatttgttctctagagc | + | |align="right"| mouse40 || sAg_bs_m10ca_fw || tcgagctcagtttactagtgacatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse41 || sAg_bs_m10ca_rev || ggccgctctagagaacaaatgtcactagtaaactgagc | + | |align="right"| mouse41 || sAg_bs_m10ca_rev || ggccgctctagagaacaaatgtcactagtaaactgagc |
|- | |- | ||
- | |align="right"| mouse42 || sAg_bs_m11gc_fw || tcgagctcagtttactagtcccatttgttctctagagc | + | |align="right"| mouse42 || sAg_bs_m11gc_fw || tcgagctcagtttactagtcccatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse43 || sAg_bs_m11gc_rev || ggccgctctagagaacaaatgggactagtaaactgagc | + | |align="right"| mouse43 || sAg_bs_m11gc_rev || ggccgctctagagaacaaatgggactagtaaactgagc |
|- | |- | ||
- | |align="right"| mouse44 || sAg_bs_m11ga_fw || tcgagctcagtttactagtaccatttgttctctagagc | + | |align="right"| mouse44 || sAg_bs_m11ga_fw || tcgagctcagtttactagtaccatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse45 || sAg_bs_m11ga_rev || ggccgctctagagaacaaatggtactagtaaactgagc | + | |align="right"| mouse45 || sAg_bs_m11ga_rev || ggccgctctagagaacaaatggtactagtaaactgagc |
|- | |- | ||
- | |align="right"| mouse46 || sAg_bs_onlyseed_fw || tcgagctcagtaatgatcacggatttgttctctagagc | + | |align="right"| mouse46 || sAg_bs_onlyseed_fw || tcgagctcagtaatgatcacggatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse47 || sAg_bs_onlyseed_rev || ggccgctctagagaacaaatccgtgatcattactgagc | + | |align="right"| mouse47 || sAg_bs_onlyseed_rev || ggccgctctagagaacaaatccgtgatcattactgagc |
|- | |- | ||
- | |align="right"| mouse48 || sAg_bs_r16-18_fw || tcgagctcagttatgtagtgccatttgttctctagagc | + | |align="right"| mouse48 || sAg_bs_r16-18_fw || tcgagctcagttatgtagtgccatttgttctctagagc |
|- | |- | ||
- | |align="right"| mouse49 || sAg_bs_r16-18_rev || ggccgctctagagaacaaatggcactacataactgagc | + | |align="right"| mouse49 || sAg_bs_r16-18_rev || ggccgctctagagaacaaatggcactacataactgagc |
|- | |- | ||
- | |align="right"| | + | |align="right"| mouse50XXX || hAAT_cDNA_SalI_Bluescript_fw || ttttgtcgacgaccatgattacgccaagcg |
|- | |- | ||
- | |align="right"| mouse51 || hAAT_cDNA_SalI_fw || ttttgtcgacgggtaccgggccccccgtcgaggatg | + | |align="right"| mouse51 || hAAT_cDNA_SalI_fw || ttttgtcgacgggtaccgggccccccgtcgaggatg |
|- | |- | ||
- | |align="right"| mouse52 || hAAT_cDNA_XhoI_NotI_NheI_rev || ttttgctagcgcggccgcgtatctcgaggctcaacccttctttaatg | + | |align="right"| mouse52 || hAAT_cDNA_XhoI_NotI_NheI_rev || ttttgctagcgcggccgcgtatctcgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse53 || hAAT_cDNA_sAg_bs_p_NheI_rev || ttttgctagcgaacaaatggcactagtaaactgaggctcaacccttctttaatg | + | |align="right"| mouse53 || hAAT_cDNA_sAg_bs_p_NheI_rev || ttttgctagcgaacaaatggcactagtaaactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse54 || hAAT_cDNA_sAg_bs_r10_12_acg_NheI_rev || ttttgctagcgaacaaatgcgtctagtaaactgaggctcaacccttctttaatg | + | |align="right"| mouse54 || hAAT_cDNA_sAg_bs_r10_12_acg_NheI_rev || ttttgctagcgaacaaatgcgtctagtaaactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse55 || hAAT_cDNA_sAg_bs_r10_12_taa_NheI_rev || ttttgctagcgaacaaatgttactagtaaactgaggctcaacccttctttaatg | + | |align="right"| mouse55 || hAAT_cDNA_sAg_bs_r10_12_taa_NheI_rev || ttttgctagcgaacaaatgttactagtaaactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse56 || hAAT_cDNA_bs_r9_12_acgg_NheI_rev || ttttgctagcgaacaaatccgtctagtaaactgaggctcaacccttctttaatg | + | |align="right"| mouse56 || hAAT_cDNA_bs_r9_12_acgg_NheI_rev || ttttgctagcgaacaaatccgtctagtaaactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse57 || hAAT_cDNA_sAg_bs_r9_12_atgt_NheI_rev || ttttgctagcgaacaaatacatctagtaaactgaggctcaacccttctttaatg | + | |align="right"| mouse57 || hAAT_cDNA_sAg_bs_r9_12_atgt_NheI_rev || ttttgctagcgaacaaatacatctagtaaactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse58 || hAAT_cDNA_sAg_bs_m10cg_NheI_rev || ttttgctagcgaacaaatgccactagtaaactgaggctcaacccttctttaatg | + | |align="right"| mouse58 || hAAT_cDNA_sAg_bs_m10cg_NheI_rev || ttttgctagcgaacaaatgccactagtaaactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse59 || hAAT_cDNA_sAg_bs_m10ca_NheI_rev || ttttgctagcgaacaaatgtcactagtaaactgaggctcaacccttctttaatg | + | |align="right"| mouse59 || hAAT_cDNA_sAg_bs_m10ca_NheI_rev || ttttgctagcgaacaaatgtcactagtaaactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse60 || hAAT_cDNA_sAg_bs_m11gc_NheI_rev || ttttgctagcgaacaaatgggactagtaaactgaggctcaacccttctttaatg | + | |align="right"| mouse60 || hAAT_cDNA_sAg_bs_m11gc_NheI_rev || ttttgctagcgaacaaatgggactagtaaactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse61 || hAAT_cDNA_sAg_bs_m11ga_NheI_rev || ttttgctagcgaacaaatggtactagtaaactgaggctcaacccttctttaatg | + | |align="right"| mouse61 || hAAT_cDNA_sAg_bs_m11ga_NheI_rev || ttttgctagcgaacaaatggtactagtaaactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse62 || hAAT_cDNA_sAg_bs_onlyseed_NheI_rev || ttttgctagcgaacaaatccgtgatcattactgaggctcaacccttctttaatg | + | |align="right"| mouse62 || hAAT_cDNA_sAg_bs_onlyseed_NheI_rev || ttttgctagcgaacaaatccgtgatcattactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| mouse63 || hAAT_cDNA_sAg_bs_r16-18_NheI_rev || ttttgctagcgaacaaatggcactacataactgaggctcaacccttctttaatg | + | |align="right"| mouse63 || hAAT_cDNA_sAg_bs_r16-18_NheI_rev || ttttgctagcgaacaaatggcactacataactgaggctcaacccttctttaatg |
|- | |- | ||
- | |align="right"| | + | |align="right"| mouse64XXX || sAg_bs_p_fw_E || aattcctcagtttactagtgccatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse65 || sAg_bs_p_rev_P || ggaacaaatggcactagtaaactgagg | + | |align="right"| mouse65 || sAg_bs_p_rev_P || ggaacaaatggcactagtaaactgagg |
|- | |- | ||
- | |align="right"| mouse66 || sAg_bs_r10_12_acg_fw_E || aattcctcagtttactagacgcatttgttcctgca | + | |align="right"| mouse66 || sAg_bs_r10_12_acg_fw_E || aattcctcagtttactagacgcatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse67 || sAg_bs_r10_12_acg_rev_P || ggaacaaatgcgtctagtaaactgagg | + | |align="right"| mouse67 || sAg_bs_r10_12_acg_rev_P || ggaacaaatgcgtctagtaaactgagg |
|- | |- | ||
- | |align="right"| mouse68 || sAg_bs_r10_12_taa_fw_E || aattcctcagtttactagtaacatttgttcctgca | + | |align="right"| mouse68 || sAg_bs_r10_12_taa_fw_E || aattcctcagtttactagtaacatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse69 || sAg_bs_r10_12_taa_rev_P || ggaacaaatgttactagtaaactgagg | + | |align="right"| mouse69 || sAg_bs_r10_12_taa_rev_P || ggaacaaatgttactagtaaactgagg |
|- | |- | ||
- | |align="right"| mouse70 || sAg_bs_r9_12_acgg_fw_E || aattcctcagtttactagacggatttgttcctgca | + | |align="right"| mouse70 || sAg_bs_r9_12_acgg_fw_E || aattcctcagtttactagacggatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse71 || sAg_bs_r9_12_acgg_rev_P || ggaacaaatccgtctagtaaactgagg | + | |align="right"| mouse71 || sAg_bs_r9_12_acgg_rev_P || ggaacaaatccgtctagtaaactgagg |
|- | |- | ||
- | |align="right"| mouse72 || sAg_bs_r9_12_atgt_fw_E || aattcctcagtttactagatgtatttgttcctgca | + | |align="right"| mouse72 || sAg_bs_r9_12_atgt_fw_E || aattcctcagtttactagatgtatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse73 || sAg_bs_r9_12_atgt_rev_P || ggaacaaatacatctagtaaactgagg | + | |align="right"| mouse73 || sAg_bs_r9_12_atgt_rev_P || ggaacaaatacatctagtaaactgagg |
|- | |- | ||
- | |align="right"| mouse74 || sAg_bs_m10cg_fw_E || aattcctcagtttactagtggcatttgttcctgca | + | |align="right"| mouse74 || sAg_bs_m10cg_fw_E || aattcctcagtttactagtggcatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse75 || sAg_bs_m10cg_rev_P || ggaacaaatgccactagtaaactgagg | + | |align="right"| mouse75 || sAg_bs_m10cg_rev_P || ggaacaaatgccactagtaaactgagg |
|- | |- | ||
- | |align="right"| mouse76 || sAg_bs_m10ca_fw_E || aattcctcagtttactagtgacatttgttcctgca | + | |align="right"| mouse76 || sAg_bs_m10ca_fw_E || aattcctcagtttactagtgacatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse77 || sAg_bs_m10ca_rev_P || ggaacaaatgtcactagtaaactgagg | + | |align="right"| mouse77 || sAg_bs_m10ca_rev_P || ggaacaaatgtcactagtaaactgagg |
|- | |- | ||
- | |align="right"| mouse78 || sAg_bs_m11gc_fw_E || aattcctcagtttactagtcccatttgttcctgca | + | |align="right"| mouse78 || sAg_bs_m11gc_fw_E || aattcctcagtttactagtcccatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse79 || sAg_bs_m11gc_rev_P || ggaacaaatgggactagtaaactgagg | + | |align="right"| mouse79 || sAg_bs_m11gc_rev_P || ggaacaaatgggactagtaaactgagg |
|- | |- | ||
- | |align="right"| mouse80 || sAg_bs_m11ga_fw_E || aattcctcagtttactagtaccatttgttcctgca | + | |align="right"| mouse80 || sAg_bs_m11ga_fw_E || aattcctcagtttactagtaccatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse81 || sAg_bs_m11ga_rev_P || ggaacaaatggtactagtaaactgagg | + | |align="right"| mouse81 || sAg_bs_m11ga_rev_P || ggaacaaatggtactagtaaactgagg |
|- | |- | ||
- | |align="right"| mouse82 || sAg_bs_onlyseed_fw_E || aattcctcagtaatgatcacggatttgttcctgca | + | |align="right"| mouse82 || sAg_bs_onlyseed_fw_E || aattcctcagtaatgatcacggatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse83 || sAg_bs_onlyseed_rev_P || ggaacaaatccgtgatcattactgagg | + | |align="right"| mouse83 || sAg_bs_onlyseed_rev_P || ggaacaaatccgtgatcattactgagg |
|- | |- | ||
- | |align="right"| mouse84 || sAg_bs_r16-18_fw_E || aattcctcagttatgtagtgccatttgttcctgca | + | |align="right"| mouse84 || sAg_bs_r16-18_fw_E || aattcctcagttatgtagtgccatttgttcctgca |
|- | |- | ||
- | |align="right"| mouse85 || sAg_bs_r16-18_rev_P || ggaacaaatggcactacataactgagg | + | |align="right"| mouse85 || sAg_bs_r16-18_rev_P || ggaacaaatggcactacataactgagg |
|} | |} | ||
+ | ==Modeling Training and Validation Binding Site Set-miRsAg== | ||
+ | |||
+ | {| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;" | ||
+ | !align="right"| ID !! Name !! Sequence (5' to 3') | ||
+ | |- | ||
+ | |align="right"| miRsAg001 || fw_model_sAg_Bs_1 || ccgggataatttgttcatttgttctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg002 || fw_model_sAg_Bs_2 || ccgggataatttgttcatttgttcatttgttctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg003 || fw_model_sAg_Bs_3 || ccgggagtttactagtgccatttgttcaaatatagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg004 || fw_model_sAg_Bs_4 || ccgggagtttactagtgcaatttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg005 || fw_model_sAg_Bs_5 || ccgggagtttactagtgaaatttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg006 || fw_model_sAg_Bs_6 || ccgggagtttactagtaaaatttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg007 || fw_model_sAg_Bs_7 || ccgggagtttactagaaaaatttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg008 || fw_model_sAg_Bs_8 || ccgggctgggcaattataaatttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg009 || fw_model_sAg_Bs_9 || ccgggctgggcagccgcaaatttgttaaaggcccgcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg010 || fw_model_sAg_Bs_10 || ccgggctgggcagctataattttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg011 || fw_model_sAg_Bs_11 || ccgggagtttacgccgtaaatttgttgaaggcccgcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg012 || fw_model_sAg_Bs_12 || ccgggctgggcaattataaatttgttgaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg013 || fw_model_sAg_Bs_13 || ccgggtccttactagtgcaatttgttaaaggcccgcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg014 || fw_model_sAg_Bs_14 || ccgggctgaatatagtgaaatttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg015 || fw_model_sAg_Bs_15 || ccgggagtttactacctaattttgttaaaatccggcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg016 || fw_model_sAg_Bs_16 || ccgggctgggcctagtggattttgttaaaggcccgcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg017 || fw_model_sAg_Bs_17 || ccgggagtttacattgcaaatttgttgaaggcccgcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg018 || fw_model_sAg_Bs_18 || ccgggctgggactagtgcaatttgttgaaggcccgcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg019 || fw_model_sAg_Bs_20 || ccgggagtttactagaaaattttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg020 || fw_model_sAg_Bs_21 || ccgggagtttactagaaaaatttgttgaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg021 || fw_model_sAg_Bs_22 || ccgggagtttactccgcaaccgctaggaaagcccgcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg022 || fw_model_sAg_Bs_23 || ccgggctgggcatagataattttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg023 || fw_model_sAg_Bs_24 || ccgggctggtactagctaattttgttaaaatccggcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg024 || fw_model_sAg_Bs_25 || ccgggagtttactagccgattttgttaaaggcccgcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg025 || fw_model_sAg_Bs_26 || ccgggctgggcctagaaaaatttgttgaaatccggcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg026 || fw_model_sAg_Bs_27 || ccgggcttttactagaaaaatttgttgaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg027 || fw_model_sAg_Bs_28 || ccgggctggtactaggcaaatttgttgaaggcccgcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg028 || fw_model_sAg_Bs_29 || ccggggctttactagaaaaatttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg029 || fw_model_sAg_Bs_30 || ccgggagtttactttaaaaatttgttaaaatttagcctctagagc | ||
+ | |- | ||
+ | |align="right"| miRsAg030 || rev_model_sAg_Bs_1 || tcgagctctagagaacaaatgaacaaattatc | ||
+ | |- | ||
+ | |align="right"| miRsAg031 || rev_model_sAg_Bs_2 || tcgagctctagagaacaaatgaacaaatgaacaaattatc | ||
+ | |- | ||
+ | |align="right"| miRsAg032 || rev_model_sAg_Bs_3 || tcgagctctagaggctatatttgaacaaatggcactagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg033 || rev_model_sAg_Bs_4 || tcgagctctagaggctaaattttaacaaattgcactagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg034 || rev_model_sAg_Bs_5 || tcgagctctagaggctaaattttaacaaatttcactagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg035 || rev_model_sAg_Bs_6 || tcgagctctagaggctaaattttaacaaattttactagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg036 || rev_model_sAg_Bs_7 || tcgagctctagaggctaaattttaacaaatttttctagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg037 || rev_model_sAg_Bs_8 || tcgagctctagaggctaaattttaacaaatttataattgcccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg038 || rev_model_sAg_Bs_9 || tcgagctctagaggcgggcctttaacaaatttgcggctgcccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg039 || rev_model_sAg_Bs_10 || tcgagctctagaggctaaattttaacaaaattatagctgcccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg040 || rev_model_sAg_Bs_11 || tcgagctctagaggcgggccttcaacaaatttacggcgtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg041 || rev_model_sAg_Bs_12 || tcgagctctagaggctaaatttcaacaaatttataattgcccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg042 || rev_model_sAg_Bs_13 || tcgagctctagaggcgggcctttaacaaattgcactagtaaggac | ||
+ | |- | ||
+ | |align="right"| miRsAg043 || rev_model_sAg_Bs_14 || tcgagctctagaggctaaattttaacaaatttcactatattcagc | ||
+ | |- | ||
+ | |align="right"| miRsAg044 || rev_model_sAg_Bs_15 || tcgagctctagaggccggattttaacaaaattaggtagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg045 || rev_model_sAg_Bs_16 || tcgagctctagaggcgggcctttaacaaaatccactaggcccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg046 || rev_model_sAg_Bs_17 || tcgagctctagaggcgggccttcaacaaatttgcaatgtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg047 || rev_model_sAg_Bs_18 || tcgagctctagaggcgggccttcaacaaattgcactagtcccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg048 || rev_model_sAg_Bs_20 || tcgagctctagaggctaaattttaacaaaattttctagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg049 || rev_model_sAg_Bs_21 || tcgagctctagaggctaaatttcaacaaatttttctagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg050 || rev_model_sAg_Bs_22 || tcgagctctagaggcgggctttcctagcggttgcggagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg051 || rev_model_sAg_Bs_23 || tcgagctctagaggctaaattttaacaaaattatctatgcccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg052 || rev_model_sAg_Bs_24 || tcgagctctagaggccggattttaacaaaattagctagtaccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg053 || rev_model_sAg_Bs_25 || tcgagctctagaggcgggcctttaacaaaatcggctagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg054 || rev_model_sAg_Bs_26 || tcgagctctagaggccggatttcaacaaatttttctaggcccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg055 || rev_model_sAg_Bs_27 || tcgagctctagaggctaaattcaacaaatttttctagtaaaagc | ||
+ | |- | ||
+ | |align="right"| miRsAg056 || rev_model_sAg_Bs_28 || tcgagctctagaggcgggccttcaacaaatttgcctagtaccagc | ||
+ | |- | ||
+ | |align="right"| miRsAg057 || rev_model_sAg_Bs_29 || tcgagctctagaggctaaattttaacaaatttttctagtaaagcc | ||
+ | |- | ||
+ | |align="right"| miRsAg058 || rev_model_sAg_Bs_30 || tcgagctctagaggctaaattttaacaaatttttaaagtaaactc | ||
+ | |- | ||
+ | |align="right"| miRsAg059 || Luc2_BS_seq_screen || acgacgatgccggcgagctg | ||
+ | |} | ||
- | |||
- | {{:Team:Heidelberg/ | + | {{:Team:Heidelberg/Single_Bottom}} |
Latest revision as of 03:59, 28 October 2010
Primer Table
Standart Kit Cloning Primers
ViroBytes primers
Primer Table Quick 'n' Dirty
Primer Table raPCR
mouse oligos
Modeling Training and Validation Binding Site Set-miRsAg
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