"
Team:Heidelberg/Notebook/miMeasure
From 2010.igem.org
(Difference between revisions)
(→miMeasure) |
|||
| (One intermediate revision not shown) | |||
| Line 92: | Line 92: | ||
| - | [[Image:MiMeasure.png|500px|miMeasure plasmid]] | + | [[Image:MiMeasure.png|500px|left|miMeasure plasmid]] |
{{:Team:Heidelberg/Single_Bottom}} | {{:Team:Heidelberg/Single_Bottom}} | ||
Latest revision as of 03:53, 28 October 2010
miMeasuremiMeasure allows to quantitatively detect endogenous miRNA in different cells. To detect miRNA one should clone binding site for it into 3' UTR of GFP gene. To ensure that transfection efficiency or amount of cells do not affect result we decided to include reference fluorescence protein, BFP to allow normalisation.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
