Team:Heidelberg/Notebook/miMeasure
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- | {{:Team:Heidelberg/ | + | {{:Team:Heidelberg/Single}} |
- | {{:Team:Heidelberg/ | + | {{:Team:Heidelberg/Single_Pagetop|note_miMeasure}} |
- | + | {{:Team:Heidelberg/Side_Top}} | |
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- | {{:Team:Heidelberg/ | + | |
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- | {| cellpadding="5" cellspacing="0" align="center | + | |
|- border="0" | |- border="0" | ||
! colspan="7" style="background:#c85000;" | [https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure/July<font color="white">July</font>] | ! colspan="7" style="background:#c85000;" | [https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure/July<font color="white">July</font>] | ||
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|colspan="3"| ||'''1'''||'''2'''||'''3'''||'''4''' | |colspan="3"| ||'''1'''||'''2'''||'''3'''||'''4''' | ||
|- style="background:#f2f2f2; color:#78b41e" | |- style="background:#f2f2f2; color:#78b41e" | ||
- | |'''5'''||'''6'''||'''7'''||'''8'''||'''9'''||'''10'''||'''11''' | + | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure/July#05.2F07.2F2010 5]'''||'''6'''||'''7'''||'''8'''||'''9'''||'''10'''||'''11''' |
|- style="background:#f2f2f2; color:#78b41e" | |- style="background:#f2f2f2; color:#78b41e" | ||
- | |'''12'''||'''13'''||'''14'''||'''15'''||'''16'''||'''17'''||'''18''' | + | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure/July#12.2F07.2F2010 12]'''||'''13'''||'''14'''||'''15'''||'''16'''||'''17'''||'''18''' |
|- style="background:#f2f2f2; color:#78b41e" | |- style="background:#f2f2f2; color:#78b41e" | ||
- | |'''19'''||'''20'''||'''21'''||'''22'''||'''23'''||'''24'''||'''25''' | + | |'''19'''||'''20'''||'''21'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure/July#22.2F07.2F2010 22]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure/July#23.2F07.2F2010 23]'''||'''24'''||'''25''' |
|- style="background:#f2f2f2; color:#78b41e" | |- style="background:#f2f2f2; color:#78b41e" | ||
- | |'''26'''||'''27'''||'''28'''||'''29'''||'''30'''||'''31'''||colspan="1"| | + | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure/July#26.2F07.2F2010 26]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure/July#27.2F07.2F2010 27]'''||'''28'''||'''29'''||'''30'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure/July#31.2F07.2F20103 1]'''||colspan="1"| |
|- style="background:#f2f2f2; color:#78b41e" | |- style="background:#f2f2f2; color:#78b41e" | ||
|colspan="7"| | |colspan="7"| | ||
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+ | {{:Team:Heidelberg/Side_Bottom}} | ||
__NOTOC__ | __NOTOC__ | ||
+ | =miMeasure= | ||
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+ | miMeasure allows to quantitatively detect endogenous miRNA in different cells. To detect miRNA one should clone binding site for it into 3' UTR of GFP gene. To ensure that transfection efficiency or amount of cells do not affect result we decided to include reference fluorescence protein, BFP to allow normalisation. | ||
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+ | [[Image:MiMeasure.png|500px|left|miMeasure plasmid]] | ||
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- | {{:Team:Heidelberg/ | + | {{:Team:Heidelberg/Single_Bottom}} |
Latest revision as of 03:53, 28 October 2010
miMeasuremiMeasure allows to quantitatively detect endogenous miRNA in different cells. To detect miRNA one should clone binding site for it into 3' UTR of GFP gene. To ensure that transfection efficiency or amount of cells do not affect result we decided to include reference fluorescence protein, BFP to allow normalisation.
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