Team:Heidelberg/Project/Capsid Shuffling/Homology Based
From 2010.igem.org
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In addition, 12 viruses were packaged with a YFP construct and used to transduce primary hepatocytes, HeLa and Huh-7 cells. Flow cytometry measurements showed that some of those viruses (e.g. number 8) are even more efficient than many of the wildtype serotypes. However, the measurements will be repeated, since not enough time was allowed for the viruses to be produced and for the cells to express sufficient YFP for good measurements. | In addition, 12 viruses were packaged with a YFP construct and used to transduce primary hepatocytes, HeLa and Huh-7 cells. Flow cytometry measurements showed that some of those viruses (e.g. number 8) are even more efficient than many of the wildtype serotypes. However, the measurements will be repeated, since not enough time was allowed for the viruses to be produced and for the cells to express sufficient YFP for good measurements. | ||
+ | Sequencing data were used for the alignment of those 12 clones with 10 AAV wildtypes (see above). | ||
Revision as of 03:52, 28 October 2010
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