Team:Heidelberg/Project/Capsid Shuffling/Homology Based
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'''Selection in Huh-7 cells:''' | '''Selection in Huh-7 cells:''' | ||
- | Three selection rounds were carried out on Huh-7 cells, the viral DNA was isolated for each selection round and cloned into a Wilson AAV8 wild-type vector. 12 clones from each selection round were picked, and viruses that contain a YFP construct were produced and used to infect 96-well plates of mouse primary hepatocytes, HeLa and Huh-7 cells. We already have some results for shuffled and selected AAV but they | + | Three selection rounds were carried out on Huh-7 cells, the viral DNA was isolated for each selection round and cloned into a Wilson AAV8 wild-type vector. 12 clones from each selection round were picked, and viruses that contain a YFP construct were produced and used to infect 96-well plates of mouse primary hepatocytes, HeLa and Huh-7 cells. We already have some results for shuffled and selected AAV but they don't represent the whole variety of clones we have. Flow cytometry measurements on the 96-well plates will follow |
[[Image:huh_clones.gif|600px]] | [[Image:huh_clones.gif|600px]] |
Revision as of 03:43, 28 October 2010
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