Team:Osaka/Notebook

From 2010.igem.org

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!align="center"|[[Team:Osaka|Home]]
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!align="center"|[[Team:Osaka/Team|Team]]
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!align="center"|[[Team:Osaka/Protocols|Protocols]]
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!align="center"|[[Team:Osaka/Notebook|Notebook]]
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!align="center"|[[Team:Osaka/Safety|Safety]]
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==Notebook==
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|{{#calendar: title=August |year=2010 | month=08}}
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Note: For detailed lab notes see individual pages in calendar above.
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.firstHeading {
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===July 29 (Thu)===
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#search-controls {
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Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura
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# Safety lecture for junior members.
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}
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# Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).
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===July 31 (Thu)===
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#footer-box {
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Attendance: Miyatake, Hirayama, Torigata, Teoh, Tadashi, Yasumoto, Kakuda, Saka
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# Meeting
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## Summer project schedule
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## List of genes to clone
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===August 2 (Mon)===
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}
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Attendance: Torigata, Takino, Teoh, Tadashi, Yasumoto, Saka
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# Culture medium preparation
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## LB agar plates (49 antibiotic-less plates)
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## LB liquid medium (500 ml)
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# Competent cells preparation - Nojima Method
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## SOB medium (MgCl2 not yet added) -> stored at 4˚C
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## TB buffer -> stored at 4˚C
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## Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n
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Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.
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===August 3 (Tue)===
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#top-section {
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Attendance: Nakamura, Kakuda, Saka, Yasumoto, Teoh
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height:25px;
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# Competent cells preparation (continued)
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width:1000px;
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## Preparation of glucose solution for making SOC medium.
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color: blue;
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## Inoculation of pre-culture SOB from o/n-incubated agar plate colonies.
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background-color: transparent;
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## (Night) Transfer from pre-culture to growth culture.
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background-image: url(https://static.igem.org/mediawiki/2010/f/f1/Osaka_top_section.jpg)
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}
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===August 4 (Wed)===
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#p-logo {
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Attendance: Nakamura, Saka, Kakuda, Yasumoto, Torigata, Teoh
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display:none;
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# OD measurements throughout the day till required OD (0.3~0.7) was obtained.
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}
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# Completion of competent cells according to protocol.
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===August 5 (Thu)===
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Attendance: Nakamura, Yasumoto, Saka, Kakuda, Takino
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# Transformation of Registry parts:
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{|
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!ID!!Part Name!!Resistance!!Description
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==Notebook==
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{| class="toccolours" style="float:{{{float|left}}}; width:{{{width|240px}}}; text-align:center;"
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|- style="background:{{{color1|#ccccff}}};"
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|colspan="8"|{{{header|July / August}}}
|-
|-
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|2-20J||<partinfo>BBa_K118023</partinfo>||A||''C. fermi'' endocellulase Cen A coding
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|- style="background:{{{color2|#eeeeff}}};"
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|2-20H||<partinfo>BBa_K118022</partinfo>||A||''C. fermi'' exocellulase Cex coding
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|<span style>[[Team:Osaka/week1|week1]]</span>
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|<span style={{{s25|"color:{{{c25|red}}};"}}}>25</span>
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|<span style={{{s26|"color:{{{c26|inherit}}};"}}}>26</span>
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|<span style={{{s27|"color:{{{c27|inherit}}};"}}}>27</span>
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|<span style={{{s28|"color:{{{c28|inherit}}};"}}}>28</span>
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|[[Team:Osaka/week1#July 29 (Thu)|29]]<span style={{{s29|"color:{{{c29|inherit}}};"}}}></span>
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|<span style={{{s30|"color:{{{c30|inherit}}};"}}}>30</span>
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|[[Team:Osaka/week1#July 31 (Sat)|31]]<span style={{{s31|"color:{{{c31|deepskyblue}}};"}}}></span>
|-
|-
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|1-2M||<partinfo>BBa_B0034</partinfo>||A||RBS
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|<span style>[[Team:Osaka/week2|week2]]</span>
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|<span style={{{s1|"color:{{{c1|red}}};"}}}>1</span>
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|[[Team:Osaka/week2#August 2 (Mon)|2]]<span style={{{s2|"color:{{{c2|inherit}}};"}}}></span>
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|[[Team:Osaka/week2#August 3 (Tue)|3]]<span style={{{s3|"color:{{{c3|inherit}}};"}}}></span>
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|[[Team:Osaka/week2#August 7 (Sat)|7]]<span style={{{s7|"color:{{{c7|deepskyblue}}};"}}}></span>
|-
|-
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|1-13D||<partinfo>BBa_B0010</partinfo>||A||Terminator
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|<span style>[[Team:Osaka/week3|week3]]</span>
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|[[Team:Osaka/week3#August 8 (Sun)|8]]<span style={{{s8|"color:{{{c8|red}}};"}}}></span>
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|[[Team:Osaka/week3#August 9 (Mon)|9]]<span style={{{s9|"color:{{{c9|inherit}}};"}}}></span>
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|[[Team:Osaka/week3#August 10 (Tue)|10]]<span style={{{s10|"color:{{{c10|inherit}}};"}}}></span>
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|[[Team:Osaka/week3#August 11 (Wed)|11]]<span style={{{s11|"color:{{{c11|inherit}}};"}}}></span>
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|<span style={{{s12|"color:{{{c12|inherit}}};"}}}>12</span>
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|<span style={{{s13|"color:{{{c13|inherit}}};"}}}>13</span>
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|<span style={{{s14|"color:{{{c14|deepskyblue}}};"}}}>14</span>
|-
|-
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|1-1D||<partinfo>BBa_R0010</partinfo>||A||Promoter
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|<span style>[[Team:Osaka/week4|week4]]</span>
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|<span style={{{s15|"color:{{{c15|red}}};"}}}>15</span>
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|[[Team:Osaka/week4#August 16 (Mon)|16]]<span style={{{s16|"color:{{{c16|inherit}}};"}}}></span>
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|[[Team:Osaka/week4#August 17 (Tue)|17]]<span style={{{s17|"color:{{{c17|inherit}}};"}}}></span>
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|[[Team:Osaka/week4#August 21 (Sat)|21]]<span style={{{s21|"color:{{{c21|deepskyblue}}};"}}}></span>
|-
|-
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|1-18F||<partinfo>BBa_E1010</partinfo>||K||RFP coding
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|<span style>[[Team:Osaka/week5|week5]]</span>
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|}
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|[[Team:Osaka/week5#August 22 (Sun)|22]]<span style={{{s22|"color:{{{c22|red}}};"}}}></span>
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Note: 'ID' is an internal identifier used by wet work members to simplify labeling etc. In the case of iGEM distribution parts it usually refers to the plate location.
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|[[Team:Osaka/week5#August 23 (Mon)|23]]<span style={{{s23|"color:{{{c23|inherit}}};"}}}></span>
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|[[Team:Osaka/week5#August 24 (Tue)|24]]<span style={{{s24|"color:{{{c24|inherit}}};"}}}></span>
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|[[Team:Osaka/week5#August 28 (Sat)|28]]<span style={{{s28|"color:{{{c28|deepskyblue}}};"}}}></span>
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|-
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|<span style>[[Team:Osaka/week6|week6]]</span>
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|[[Team:Osaka/week6#August 29 (Sun)|29]]<span style={{{s29|"color:{{{c29|red}}};"}}}></span>
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|[[Team:Osaka/week6#August 30 (Mon)|30]]<span style={{{s30|"color:{{{c30|inherit}}};"}}}></span>
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|[[Team:Osaka/week6#August 31 (Tue)|31]]<span style={{{s31|"color:{{{c31|inherit}}};"}}}></span>
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|colspan="4"|
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|- style="background:{{{color3|#eeeeff}}};"
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|colspan="7"|{{{footer|}}}
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|}<noinclude>
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{| class="toccolours" style="float:{{{float|left}}}; width:{{{width|240px}}}; text-align:center;"
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|- style="background:{{{color1|#ccccff}}};"
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|colspan="8"|{{{header|September}}}
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|-
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|-
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|<span style>[[Team:Osaka/week6|week6]]</span>
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|[[Team:Osaka/week6#September 1 (Wed)|1]]<span style={{{s1|"color:{{{c1|inherit}}};"}}}></span>
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|-
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|[[Team:Osaka/week7#September 5 (Sun)|5]]<span style={{{s5|"color:{{{c5|red}}};"}}}></span>
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|-
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|<span style>[[Team:Osaka/week8|week8]]</span>
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|[[Team:Osaka/week8#September 12 (Sun)|12]]<span style={{{s12|"color:{{{c12|red}}};"}}}></span>
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|[[Team:Osaka/week8#September 13 (Mon)|13]]<span style={{{s13|"color:{{{c13|inherit}}};"}}}></span>
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|[[Team:Osaka/week8#September 14 (Tue)|14]]<span style={{{s14|"color:{{{c14|inherit}}};"}}}></span>
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|[[Team:Osaka/week8#September 15 (Wed)|15]]<span style={{{s15|"color:{{{c15|inherit}}};"}}}></span>
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|[[Team:Osaka/week8#September 16 (Thu)|16]]<span style={{{s16|"color:{{{c16|inherit}}};"}}}></span>
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|[[Team:Osaka/week8#September 18 (Sat)|18]]<span style={{{s18|"color:{{{c18|deepskyblue}}};"}}}></span>
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|-
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|<span style>[[Team:Osaka/week9|week9]]</span>
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|[[Team:Osaka/week9#September 19 (Sun)|19]]<span style={{{s19|"color:{{{c19|red}}};"}}}></span>
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|[[Team:Osaka/week9#September 20 (Mon)|20]]<span style={{{s20|"color:{{{c20|inherit}}};"}}}></span>
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|[[Team:Osaka/week9#September 23 (Thu)|23]]<span style={{{s23|"color:{{{c23|inherit}}};"}}}></span>
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|[[Team:Osaka/week9#September 24 (Fri)|24]]<span style={{{s24|"color:{{{c24|inherit}}};"}}}></span>
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|[[Team:Osaka/week9#September 25 (Sat)|25]]<span style={{{s25|"color:{{{c25|deepskyblue}}};"}}}></span>
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|-
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|<span style>[[Team:Osaka/week10|week10]]</span>
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|[[Team:Osaka/week10#September 26 (Sun)|26]]<span style={{{s26|"color:{{{c26|red}}};"}}}></span>
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|[[Team:Osaka/week10#September 27 (Mon)|27]]<span style={{{s27|"color:{{{c27|inherit}}};"}}}></span>
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|[[Team:Osaka/week10#September 28 (Tue)|28]]<span style={{{s28|"color:{{{c28|inherit}}};"}}}></span>
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|[[Team:Osaka/week10#September 29 (Wed)|29]]<span style={{{s29|"color:{{{c29|inherit}}};"}}}></span>
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|[[Team:Osaka/week10#September 30 (Thu)|30]]<span style={{{s30|"color:{{{c30|inherit}}};"}}}></span>
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|colspan="2"|
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|- style="background:{{{color3|#eeeeff}}};"
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|colspan="7"|{{{footer|}}}
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|}<noinclude>
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{| class="toccolours" style="float:{{{float|left}}}; width:{{{width|240px}}}; text-align:center;"
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|- style="background:{{{color1|#ccccff}}};"
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|colspan="8"|{{{header|October}}}
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|-
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|- style="background:{{{color2|#eeeeff}}};"
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|[[Team:Osaka/week10#October 1 (Fri)|1]]<span style={{{s1|"color:{{{c1|inherit}}};"}}}></span>
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|[[Team:Osaka/week10#October 2 (Sat)|2]]<span style={{{s2|"color:{{{c2|deepskyblue}}};"}}}></span>
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|-
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|<span style>[[Team:Osaka/week11|week11]]</span>
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|[[Team:Osaka/week11#October 3 (Sun)|3]]<span style={{{s3|"color:{{{c3|red}}};"}}}></span>
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|[[Team:Osaka/week11#October 4 (Mon)|4]]<span style={{{s4|"color:{{{c4|inherit}}};"}}}></span>
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|[[Team:Osaka/week11#October 6 (Wed)|6]]<span style={{{s6|"color:{{{c6|inherit}}};"}}}></span>
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|-
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|<span style>[[Team:Osaka/week12|week12]]</span>
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|[[Team:Osaka/week12#October 10 (Sun)|10]]<span style={{{s10|"color:{{{c10|red}}};"}}}></span>
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|[[Team:Osaka/week12#October 11 (Mon)|11]]<span style={{{s11|"color:{{{c11|inherit}}};"}}}></span>
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|[[Team:Osaka/week12#October 12 (Tue)|12]]<span style={{{s12|"color:{{{c12|inherit}}};"}}}></span>
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|[[Team:Osaka/week12#October 13 (Wed)|13]]<span style={{{s13|"color:{{{c13|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week12#October 14 (Thu)|14]]<span style={{{s14|"color:{{{c14|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week12#October 15 (Fri)|15]]<span style={{{s15|"color:{{{c15|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week12#October 16 (Sat)|16]]<span style={{{s16|"color:{{{c16|deepskyblue}}};"}}}></span>
 +
|-
 +
|<span style>[[Team:Osaka/week13|week13]]</span>
 +
|[[Team:Osaka/week13#October 17 (Sun)|17]]<span style={{{s17|"color:{{{c17|red}}};"}}}></span>
 +
|[[Team:Osaka/week13#October 18 (Mon)|18]]<span style={{{s18|"color:{{{c18|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week13#October 19 (Tue)|19]]<span style={{{s19|"color:{{{c19|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week13#October 20 (WEd)|20]]<span style={{{s20|"color:{{{c20|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week13#October 21 (Thu)|21]]<span style={{{s21|"color:{{{c21|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week13#October 22 (Fri)|22]]<span style={{{s22|"color:{{{c22|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week13#October 23 (Sat)|23]]<span style={{{s23|"color:{{{c23|deepskyblue}}};"}}}></span>
 +
|-
 +
|<span style>[[Team:Osaka/week14|week14]]</span>
 +
|[[Team:Osaka/week14#October 24 (Sun)|24]]<span style={{{s24|"color:{{{c24|red}}};"}}}></span>
 +
|[[Team:Osaka/week14#October 25 (Mon)|25]]<span style={{{s25|"color:{{{c25|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week14#October 26 (Tue)|26]]<span style={{{s26|"color:{{{c26|inherit}}};"}}}></span>
 +
|[[Team:Osaka/week14#October 27 (Wed)|27]]<span style={{{s27|"color:{{{c27|inherit}}};"}}}></span>
 +
|<span style={{{s28|"color:{{{c28|inherit}}};"}}}>28</span>
 +
|<span style={{{s29|"color:{{{c29|inherit}}};"}}}>29</span>
 +
|<span style={{{s30|"color:{{{c30|deepskyblue}}};"}}}>30</span>
 +
|-
 +
|<span style>[[Team:Osaka/week15|week15]]</span>
 +
|[[{{{prefix|}}}10.31{{{suffix|}}}|<span style={{{s31|"color:{{{c31|red}}};"}}}>31</span>]]
 +
|colspan="6"|
 +
|- style="background:{{{color3|#eeeeff}}};"
 +
|colspan="7"|{{{footer|}}}
 +
|}<noinclude>
 +
 
 +
 
-
===August 6 (Fri)===
 
-
# Colony check
 
-
## All transformed cells produced colonies!
 
-
## Non-transformed cells (negative controls) did not grow on Amp, Kan or Cam plates -> confirmed lack of natural antibiotic resistance
 
-
# Colonies transferred to LB growth medium & incubated o/n at 37˚C
 
-
===August 7 (Sat)===
 
-
# Miniprep
 
-
# Transformation
 
-
# Meeting
 
-
===August 8 (Sun)===
+
<html>
-
# Transfer to liquid culture medium
+
</div>
 +
</div>
 +
</div>
 +
<div class="clear"><hr></div>
 +
</html>
 +
{{Osaka_foot}}
 +
<html>
-
===August 9 (Mon)===
+
</html>
-
# Assembly
+
-
# Miniprep
+
-
# Transfer to liquid culture medium (repeat)
+

Latest revision as of 03:34, 28 October 2010

Notebook

July / August
Week S M T W T F S
week1 25 26 27 28 29 30 31
week2 1 2 3 4 5 6 7
week3 8 9 10 11 12 13 14
week4 15 16 17 18 19 20 21
week5 22 23 24 25 26 27 28
week6 29 30 31
September
Week S M T W T F S
week6 1 2 3 4
week7 5 6 7 8 9 10 11
week8 12 13 14 15 16 17 18
week9 19 20 21 22 23 24 25
week10 26 27 28 29 30
October
Week S M T W T F S
week10 1 2
week11 3 4 5 6 7 8 9
week12 10 11 12 13 14 15 16
week13 17 18 19 20 21 22 23
week14 24 25 26 27 28 29 30
week15 31



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Retrieved from "http://2010.igem.org/Team:Osaka/Notebook"