Team:Chiba/Notebook 3

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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/Result"><span>Result</span></a></li>
 
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li>
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<br><br><br><br>
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<font size="5">Characterization of ''lux'' inverter</font><br>
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<font size="5">Characterization of lux inverter</font><br><br>
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<html><h3>2010-10-8</h3></html>
<html><h3>2010-10-8</h3></html>
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<pre>transformation
<pre>transformation
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<pre>
<pre>
liquid culture
liquid culture
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LB (Carb-Kan) 1mL
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Sample:Plux inv-GFP and Plac-LuxR
 +
 
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LB (Carb-Kan,AHL 0 nM and 1000 nM ) 1mL
37℃ 12 h 200 rpm
37℃ 12 h 200 rpm
</pre>
</pre>
<html><h3>2010-10-26</h3></html>
<html><h3>2010-10-26</h3></html>
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<pre>
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Spin-dawn and observed the pellet
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</pre>
　
　

Latest revision as of 03:21, 28 October 2010




 

 





Characterization of lux inverter

2010-10-8

transformation

Plasmid:BBa_R0061
        BBa_E0240
strain:XL10-G


2010-10-9

Mini-prep (Sigma Aldrich)

BBa_R0061
BBa_E0240

digestion
--------------------
BBa_R0061      20 µL
10×Buffer      3 µL
SpeI　　　　　  1 µL
PstI　　　　　  1 µL
NFW             5 µL
--------------------
　　　　　　　 30 µL

--------------------
BBa_E0240      20 µL
10×Buffer      3 µL
XbaI            1 µL
PstI　　　　　  1 µL
NFW             5 µL
--------------------
               30 µL
↓
gel extraction


2010-10-11

Ligation
-----------------------
BBa_R0061 (70 ng)  1 µL       
BBa_E0240 (100 ng) 2 µL
ATP (10 mM)        1 µL
10×Buffer         1 µL
T4 DNA Ligase      1 µL
NFW                4 µL
-----------------------
                  10 µL
↓
transformation
strain:XL10-G

2010-10-12

Ligation
-----------------------
BBa_R0061(100 ng)  1 µL
BBa_E0240 (100 ng) 3 µL
ATP (10 mM)        1 µL
T4 DNA Ligase      1 µL
dH<sub>2</sub>O    4 µL
-----------------------
                  10 µL
↓
transformation
strain:XL10-Gold


2010-10-21

Double transformation

Strain:DH10B
Plasmid:Plux inv-GFP & Plac-LuxR

2010-10-22

liquid culture
Sample:Plux inv-GFP and Plac-LuxR

LB (Carb-Kan,AHL 0 nM and 1000 nM ) 1mL
37℃ 12 h 200 rpm

2010-10-26

Spin-dawn and observed the pellet

　

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