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Week of 9/27
From 2010.igem.org
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An extension time of 1 hour and 5 minutes was used. | An extension time of 1 hour and 5 minutes was used. | ||
| - | + | <center>[[Image:9-27-10.jpg]]</center> | |
==September 30th== | ==September 30th== | ||
| - | + | ||
| + | The oxygen sensing promoters were run today! | ||
| + | |||
| + | Gel order: | ||
| + | -ladder | ||
| + | -D1 | ||
| + | -D2 | ||
| + | -D3 | ||
| + | -D4 | ||
| + | -D5 | ||
| + | -J6 | ||
| + | -J7 | ||
| + | -J8 | ||
| + | -J9 | ||
| + | -J10 | ||
| + | <center>[[Image:9-30-10.jpg]]</center> | ||
| + | |||
| + | |||
| + | ==October 2nd== | ||
| + | |||
| + | After experiencing some minor problems, we re-ran the oxygen sensitive promoters. This time around we had great success!! | ||
| + | |||
| + | *Gel Order: | ||
| + | -Ladder | ||
| + | -D1 | ||
| + | -D2 | ||
| + | -D3 | ||
| + | -D4 | ||
| + | -D5 | ||
| + | -J6 | ||
| + | -J7 | ||
| + | -J8 | ||
| + | -J9 | ||
| + | -J10 | ||
| + | |||
| + | <center>[[Image:10-02-10.jpg]]</center> | ||
[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | ||
Latest revision as of 03:19, 28 October 2010
September 29th
- Colony PCR:
-LuxR+J23100 -LuxR+J23108 -LuxR+J23116
An extension time of 1 hour and 5 minutes was used.
September 30th
The oxygen sensing promoters were run today!
Gel order:
-ladder -D1 -D2 -D3 -D4 -D5 -J6 -J7 -J8 -J9 -J10
October 2nd
After experiencing some minor problems, we re-ran the oxygen sensitive promoters. This time around we had great success!!
- Gel Order:
-Ladder -D1 -D2 -D3 -D4 -D5 -J6 -J7 -J8 -J9 -J10
