Team:Heidelberg/Notebook/BSDesign/July
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{{:Team:Heidelberg/Single_Pagetop|note_BSDesign}} | {{:Team:Heidelberg/Single_Pagetop|note_BSDesign}} | ||
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+ | {| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#4e93a4; border:1.53px solid #333333;" | ||
+ | |- border="0" | ||
+ | ! colspan="7" style="background:#c85000;" | [https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July<font color="white">July</font>] | ||
+ | |- style="background:#c85000; color:white" | ||
+ | |width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |colspan="3"| ||'''1'''||'''2'''||'''3'''||'''4''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#05.2F07.2F2010_-_11.2F07.2F2010 5]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#05.2F07.2F2010_-_11.2F07.2F2010 6]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#05.2F07.2F2010_-_11.2F07.2F2010 7]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#05.2F07.2F2010_-_11.2F07.2F2010 8]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#05.2F07.2F2010_-_11.2F07.2F2010 9]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#05.2F07.2F2010_-_11.2F07.2F2010 10]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#05.2F07.2F2010_-_11.2F07.2F2010 11]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#12.2F07.2F2010_-_19.2F07.2F2010 12]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#12.2F07.2F2010_-_19.2F07.2F2010 13]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#12.2F07.2F2010_-_19.2F07.2F2010 14]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#12.2F07.2F2010_-_19.2F07.2F2010 15]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#12.2F07.2F2010_-_19.2F07.2F2010 16]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#12.2F07.2F2010_-_19.2F07.2F2010 17]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#12.2F07.2F2010_-_19.2F07.2F2010 18]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#12.2F07.2F2010_-_19.2F07.2F2010 19]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#20.2F07.2F2010 20]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#21.2F07.2F2010 21]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#22.2F07.2F2010 22]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#23.2F07.2F2010 23]'''||'''24'''||'''25''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#26.2F07.2F2010 26]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#27.2F07.2F2010 27]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#28.2F07.2F2010 28]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#29.2F07.2F2010 29]'''||'''30'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/July#31.2F07.2F2010 31]'''|| | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | | colspan="7"| | ||
+ | <span style="color:#ffffff">-</span> | ||
+ | |} | ||
+ | |||
+ | |||
+ | {| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#f09600; border: 1.5px solid #000000;" | ||
+ | |- border="0" | ||
+ | ! colspan="7" style="background:#f09600;" | [https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August<font color="white">August</font>] | ||
+ | |- style="background:#f09600; color:white" | ||
+ | |width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |colspan="6"| ||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#01.2F08.2F2010 1]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#02.2F08.2F2010 2]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#03.2F08.2F2010 3]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#04.2F08.2F2010 4]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#05.2F08.2F2010 5]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#06.2F08.2F2010 6]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#07.2F08.2F2010 7]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#08.2F08.2F2010 8]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#09.2F08.2F2010 9]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#10.2F08.2F2010 10]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/August#11.2F08.2F2010 11]'''||'''12'''||'''13'''||'''14'''||'''15''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''16'''||'''17'''||'''18'''||'''19'''||'''20'''||'''21'''||'''22''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''23'''||'''24'''||'''25'''||'''26'''||'''27'''||'''28'''||'''29''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''30'''||'''31'''||colspan="5"| | ||
+ | |} | ||
+ | |||
+ | |||
+ | {| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#f09600; border: 1.5px solid #333333;" | ||
+ | |- border="0" | ||
+ | ! colspan="7" style="background:#009be1;" | [https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September<font color="#ffecba">September</font>] | ||
+ | |- style="background:#009be1; color:white" | ||
+ | |width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |colspan="2"| ||'''1'''||'''2'''||'''3'''||'''4'''||'''5''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''6'''||'''7'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#08.2F09.2F2010 8]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#09.2F09.2F2010 9]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#10.2F09.2F2010 10]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#11.2F09.2F2010 11]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#12.2F09.2F2010 12]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#13.2F09.2F2010 13]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#14.2F09.2F2010 14]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#15.2F09.2F2010 15]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#16.2F09.2F2010 16]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#17.2F09.2F2010 17]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#18.2F09.2F2010 18]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#19.2F09.2F2010 19]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#20.2F09.2F2010 20]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#21.2F09.2F2010 21]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#22.2F09.2F2010 22]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#23.2F09.2F2010 23]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#24.2F09.2F2010 24]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#25.2F09.2F2010 25]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#26.2F09.2F2010 26]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#27.2F09.2F2010 27]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#28.2F09.2F2010 28]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#29.2F09.2F2010 29]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/September#30.2F30.2F2010 30]'''||colspan="5"| | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |colspan="7"| | ||
+ | <span style="color:#ffffff">-</span> | ||
+ | |} | ||
+ | |||
+ | |||
+ | {| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#f09600; border: 1.5px solid #333333;" | ||
+ | |- border="0" | ||
+ | ! colspan="7" style="background:#78b41e;" | [https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October<font color="white">October</font>] | ||
+ | |- style="background:#78b41e; color:white" | ||
+ | |width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |colspan="4"| ||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#01.2F10.2F2010 1]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#02.2F10.2F2010 2]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#03.2F10.2F2010 3]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#04.2F10.2F2010 4]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#05.2F10.2F2010 5]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#06.2F10.2F2010 6]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#07.2F10.2F2010 7]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#08.2F10.2F2010 8]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#09.2F10.2F2010 9]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#10.2F10.2F2010 10]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#11.2F10.2F2010 11]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#12.2F10.2F2010 12]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#13.2F10.2F2010 13]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#14.2F10.2F2010 14]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#15.2F10.2F2010 15]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#16.2F10.2F2010 16]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#17.2F10.2F2010 17]''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#18.2F10.2F2010 18]'''|||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#19.2F10.2F2010 19]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#20.2F10.2F2010 20]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#21.2F10.2F2010 21]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#22.2F10.2F2010 22]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October#23.2F10.2F2010 23]'''||'''24''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |'''25'''||'''26'''||'''27'''||'''28'''||'''29'''||'''30'''||'''31''' | ||
+ | |- style="background:#f2f2f2; color:#f09600" | ||
+ | |colspan="7"| | ||
+ | <span style="color:#ffffff">-</span> | ||
+ | |} | ||
{{:Team:Heidelberg/Side_Bottom}} | {{:Team:Heidelberg/Side_Bottom}} |
Latest revision as of 03:17, 28 October 2010
Binding Site Design - July05/07/2010 - 11/07/2010
Preperation of chemically competent cells E. coli Top10 and DH5alpha
First, a 20 ml over night culture was inoculated in antibiotic free LB medium from a fresh single colony and transferred into 400 ml antibiotic free LB medium the next day. This culture was incubated at 37 °C while shacking until an OD600 of 0.5 – 0.6 was achieved. The culture was than cooled down on ice, centrifuged (8 min, 4 °C, 3500 rpm), the supernatant discarded and the pellet resuspended in 10 ml 100 mM CaCl2. After addition of further 190 ml 100 mM CaCl2 the suspension was incubated on ice for 30 min. The suspension was than again centrifuged (8 min, 4 °C, 3500 rpm), the supernatant discarded, the pellet resuspended in 20 ml 82.5 mM CaCl2 with 17.5 % glycerol and aliquoted. The aliquots were flash frozen in liquid nitrogen and than stored at -80 °C until usage.
Cell Culture Starting
Media for HUH-7 cells:
12/07/2010 - 19/07/2010
Preperation of RNA extracts for miRNA profiling
Protocol
design of diraPCR oligos
design of measurement standard
please, find more information about the measurement standard design in the measurment standard page
20/07/2010
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21/07/2010
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22/07/2010
23/07/2010
26/07/2010
single binding site synthesis
PCR protocol:
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27/07/2010
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Single Binding Site Synthesis - Optimization
28/07/2010
raPCR for the construction of anti miRsAg-shRNA sponges
31/07/2010
diraPCR for constructing hsa-886-3p binding site patterns
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