Team:St Andrews

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Welcome to the the University of St Andrews iGEM wiki. This is our first year participating in iGEM and we're very excited to be here! This page is constantly changing, check back for more soon!
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Welcome to the the University of St Andrews <a href="https://2010.igem.org/Main_Page"> iGEM 2010</a> Team Website. We are the first University of St Andrews iGEM Team. Our work is mainly based on Quorum Sensing and trying to investigate possible applications in Synthetic Biology.
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<td width="40%"> We're St Andrews' first iGEM team. Comprised of nine studenents and assisted by five advisors we represent a number of different scientific fields. Working on several exciting subprojects and on our main challenge we have been busy getting to grips with the incredible world of igem and synthetic biology. <a href="https://2010.igem.org/Team:St Andrews/team/members"> Meet our Team</a> and <a href="https://2010.igem.org/Team:St Andrews/advisors">our advisors!</a></td>
 
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<td>Cholera is a deadly infection caused by the bacterium Vibrio cholerae which causes massive dehydration and electrolyte loss. Predominantly effecting = the deveolping world Cholera is one of the world's greatest killers. Find out how through the use of quorum sensing and synthetic biology we propose a countermeasure to stop Cholera in it's tracks. <a href="https://2010.igem.org/Team:St Andrews/project/objectives"> Find out more about our project. </a></td>
 
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<td width="260"> Comprised of 9 undergraduate <a href="https://2010.igem.org/Team:St Andrews/team/members">students</a> and guided by 5 <a href="https://2010.igem.org/Team:St Andrews/advisors">advisors</a>, our team stems from a variety of different scientific fields: Medicine, Biological Sciences, Chemistry, Computer Science and Physics.</td>
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<td>Cholera is a bacterial disease that infects approximately 5 million people worldwide each year, approximately 100,000 of which are fatal. Symptoms of an acute cholera infection including diarrhoea and severe dehydration that can kill within hours if left untreated. <br/>
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Provision of safe water is critical to preventing cholera outbreaks however in many cases this is not feasible, particularly in areas recently hit by natural disaster. Our project involves investigating the basic science behind a potential means of preventing the disease through the application of synthetic biology. Lately Cholera outbreaks occurred in Pakistan and Haiti. In Haiti around 3500 confirmed causes have been reported <a href="http://www.elpais.com/articulo/internacional/Haiti/lucha/reloj/frenar/epidemia/colera/elpepiint/20101025elpepiint_5/Tes">news in spanish</a> and <a href="http://www.bbc.co.uk/news/world-latin-america-11632738">news in english</a>. Read more about our <a href="https://2010.igem.org/Team:St Andrews/project/objectives"> project</a>. </td>
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<td width="260"> We would like to thank our <a href="https://2010.igem.org/Team:St Andrews/team/sponsors">sponsors </a>: multiple faculties of the University of St Andrews, biotech companies, etc., whose generosity has made this all possible.</td>
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<td>We would like to offer our greatest thanks to all our sponsors, your generosity has allowed us to make all this possible. For a full list of our sponsors, both from St Andrews and around the world please visit <a href="https://2010.igem.org/Team:St Andrews/sponsors"> here </a></td>
 
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To better understand the inner workings of the Cholera quorum sensing system we produced a series of computational models to simulate the operation of Cholera. Based upon differential equations and solved computationally via the runge kutta method our models provide a comprehensive view of Cholera quorum sensing and bi-stable switching behaviour. To find out how we designed our models from the blackboard to the CPU check out our <a href="https://2010.igem.org/Team:St Andrews/project/modelling"> modelling pages </a>.
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Part of our project is also to help other iGEM teams (both current and future) by fine-tunning the control over protein expression by designing new ribosome binding sites.
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We collaborated in iGEM 2010 with multiple teams in multiple contexts (wiki development (<a href="https://2010.igem.org/Team:TU_Delft">Delft</a> team), human practices data collection, exhange of DNA (<a href="https://2010.igem.org/Team:Sheffield">Sheffield</a> team)...).
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Over the summer months our team have been hard at work doing things that the person writing this wiki has no idea about, running gels, PCR, catogarising stuff, making biobricks, creating fire etc. To find out more check out our <a href="https://2010.igem.org/Team:St Andrews/project/laboratory"> wet lab section </a>
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In order to complete our project several biobricks must be constructed. Through use of standard protocols and procedures we plan to construct a bistable switch based on the Lux quorum sensing system and a CAI-1 sender using the cqsa gene from <i>Vibrio cholerae</i>. Follow our progress in the<a href="https://2010.igem.org/Team:St Andrews/project/laboratory"> laboratory. </a>
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<a href="https://2010.igem.org/Team:St Andrews/project/ethics">Human Practices</a> shapes the future and the very being of all science. Human Practices includes (but is not limited to) the purpose, effects and impact of science on society. A realm where ethics, economics and <i>E.coli</i> all intertwine.
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Integral to iGEM and all scientific endevour are human practices. Human pratices is an expansive term encorporating ethics, group dynamics, sharing and cooperation. Part philosophy and part psychology human practices is all about the people involved in science, from those behind it to those effected by it, how all these parties interact and how they can learn from each other. To find out about the ethical and social issues we considered and how we see the future of synthetic biology and iGEM vist our <a href="https://2010.igem.org/Team:St Andrews/project/ethics"> human practices page </a>.
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To better understand the inner workings of the <i>Vibrio cholera</i> quorum sensing system we produced a series of computational models to simulate the operation of <i>Vibrio cholerae</i>. Based upon differential equations and solved computationally via the Fourth Order Runge-Kutta method our models provide a comprehensive view of <i>Vibrio cholerae</i> quorum sensing and bi-stable switching behaviour. To find out how we designed our models from the blackboard to the CPU check out our <a href="https://2010.igem.org/Team:St Andrews/project/modelling">modelling </a>. This work is potentially a flexible framework for future quorum sensing modelling. Possible future extensions can be other diffusion models for quorum sensing, other quorum sensing systems, etc. Different models are proposed as an example.</td>
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We proposed a new solution for the fine-tuning the translation of proteins designing Ribosome Binding Site by the usage of an RBS calculator. Check out more about this <a href="https://2010.igem.org/Team:St_Andrews/project/RBS">RBS</a>.
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* [[Team:St_Andrews/FAQ | Frequently Asked Questions ]]
* [[Team:St_Andrews/FAQ | Frequently Asked Questions ]]
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* [https://igem.org/Main_Page iGEM 2010 ]

Latest revision as of 03:02, 28 October 2010


St Andrews from East Sands

University of St Andrews iGEM 2010

Welcome!

The Saints

University of St Andrews iGEM 2010

Our first year at iGEM!


University of St Andrews 2010 iGEM Team

Welcome to the the University of St Andrews iGEM 2010 Team Website. We are the first University of St Andrews iGEM Team. Our work is mainly based on Quorum Sensing and trying to investigate possible applications in Synthetic Biology.

Comprised of 9 undergraduate students and guided by 5 advisors, our team stems from a variety of different scientific fields: Medicine, Biological Sciences, Chemistry, Computer Science and Physics. Cholera is a bacterial disease that infects approximately 5 million people worldwide each year, approximately 100,000 of which are fatal. Symptoms of an acute cholera infection including diarrhoea and severe dehydration that can kill within hours if left untreated.
Provision of safe water is critical to preventing cholera outbreaks however in many cases this is not feasible, particularly in areas recently hit by natural disaster. Our project involves investigating the basic science behind a potential means of preventing the disease through the application of synthetic biology. Lately Cholera outbreaks occurred in Pakistan and Haiti. In Haiti around 3500 confirmed causes have been reported news in spanish and news in english. Read more about our project. 		We would like to thank our sponsors : multiple faculties of the University of St Andrews, biotech companies, etc., whose generosity has made this all possible.
Part of our project is also to help other iGEM teams (both current and future) by fine-tunning the control over protein expression by designing new ribosome binding sites. We collaborated in iGEM 2010 with multiple teams in multiple contexts (wiki development (Delft team), human practices data collection, exhange of DNA (Sheffield team)...).
In order to complete our project several biobricks must be constructed. Through use of standard protocols and procedures we plan to construct a bistable switch based on the Lux quorum sensing system and a CAI-1 sender using the cqsa gene from Vibrio cholerae. Follow our progress in the laboratory.
Human Practices shapes the future and the very being of all science. Human Practices includes (but is not limited to) the purpose, effects and impact of science on society. A realm where ethics, economics and E.coli all intertwine. To better understand the inner workings of the Vibrio cholera quorum sensing system we produced a series of computational models to simulate the operation of Vibrio cholerae. Based upon differential equations and solved computationally via the Fourth Order Runge-Kutta method our models provide a comprehensive view of Vibrio cholerae quorum sensing and bi-stable switching behaviour. To find out how we designed our models from the blackboard to the CPU check out our modelling . This work is potentially a flexible framework for future quorum sensing modelling. Possible future extensions can be other diffusion models for quorum sensing, other quorum sensing systems, etc. Different models are proposed as an example. We proposed a new solution for the fine-tuning the translation of proteins designing Ribosome Binding Site by the usage of an RBS calculator. Check out more about this RBS.

Retrieved from "http://2010.igem.org/Team:St_Andrews"