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| - | <html>
| + | {{:Team:St_Andrews/defaulttemplate}} |
| - | <head>
| + | |
| - | <style type="text/css">
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| - | #globalWrapper {
| + | <html><h1>University of St Andrews 2010 iGEM Team</h1> |
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| - | #top-section {
| + | <p> |
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| + | Welcome to the the University of St Andrews <a href="https://2010.igem.org/Main_Page"> iGEM 2010</a> Team Website. We are the first University of St Andrews iGEM Team. Our work is mainly based on Quorum Sensing and trying to investigate possible applications in Synthetic Biology. |
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| + | </p> |
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| + | <p> |
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| + | <td width="260"> Comprised of 9 undergraduate <a href="https://2010.igem.org/Team:St Andrews/team/members">students</a> and guided by 5 <a href="https://2010.igem.org/Team:St Andrews/advisors">advisors</a>, our team stems from a variety of different scientific fields: Medicine, Biological Sciences, Chemistry, Computer Science and Physics.</td> |
| - | position: absolute;
| + | <td>Cholera is a bacterial disease that infects approximately 5 million people worldwide each year, approximately 100,000 of which are fatal. Symptoms of an acute cholera infection including diarrhoea and severe dehydration that can kill within hours if left untreated. <br/> |
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| | + | Provision of safe water is critical to preventing cholera outbreaks however in many cases this is not feasible, particularly in areas recently hit by natural disaster. Our project involves investigating the basic science behind a potential means of preventing the disease through the application of synthetic biology. Lately Cholera outbreaks occurred in Pakistan and Haiti. In Haiti around 3500 confirmed causes have been reported <a href="http://www.elpais.com/articulo/internacional/Haiti/lucha/reloj/frenar/epidemia/colera/elpepiint/20101025elpepiint_5/Tes">news in spanish</a> and <a href="http://www.bbc.co.uk/news/world-latin-america-11632738">news in english</a>. Read more about our <a href="https://2010.igem.org/Team:St Andrews/project/objectives"> project</a>. </td> |
| | | | |
| | + | <td width="260"> We would like to thank our <a href="https://2010.igem.org/Team:St Andrews/team/sponsors">sponsors </a>: multiple faculties of the University of St Andrews, biotech companies, etc., whose generosity has made this all possible.</td> |
| | | | |
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| + | <td> |
| - | background: -webkit-gradient(linear, left top, left bottom, from(#ebebeb), to(#808080)); /* for webkit browsers */
| + | Part of our project is also to help other iGEM teams (both current and future) by fine-tunning the control over protein expression by designing new ribosome binding sites. |
| - | background: -moz-linear-gradient(top, #ebebeb, #808080); /* for firefox 3.6+ */
| + | We collaborated in iGEM 2010 with multiple teams in multiple contexts (wiki development (<a href="https://2010.igem.org/Team:TU_Delft">Delft</a> team), human practices data collection, exhange of DNA (<a href="https://2010.igem.org/Team:Sheffield">Sheffield</a> team)...). |
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| + | <img src="http://i.imgur.com/L07jJ.jpg"/><br/> |
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| - | html>body #nav {
| + | <td> |
| - | filter: alpha(opacity=80);
| + | In order to complete our project several biobricks must be constructed. Through use of standard protocols and procedures we plan to construct a bistable switch based on the Lux quorum sensing system and a CAI-1 sender using the cqsa gene from <i>Vibrio cholerae</i>. Follow our progress in the<a href="https://2010.igem.org/Team:St Andrews/project/laboratory"> laboratory. </a> |
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| + | </td> |
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| - |
| + | <tr> |
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| + | <td> |
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| + | <a href="https://2010.igem.org/Team:St Andrews/project/ethics">Human Practices</a> shapes the future and the very being of all science. Human Practices includes (but is not limited to) the purpose, effects and impact of science on society. A realm where ethics, economics and <i>E.coli</i> all intertwine. |
| - |
| + | </td> |
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| - | background: -webkit-gradient(linear, left top, left bottom, from(#000066), to(#000000)); /* for webkit browsers */
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| - | background: -moz-linear-gradient(top, #000066, #000000); /* for firefox 3.6+ */
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| - | border: solid 1px #6d6d6d;
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| - | }
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| - | html>body #nav li {
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| - | margin: 0 5px;
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| - | padding: 0 0 8px;
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| - | float: left;
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| - | position: relative;
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| - | list-style: none;
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| - | }
| + | |
| - | /* main level link */ | + | |
| - | html>body #nav a {
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| + | <td> |
| - | html>body #nav .current a, #nav li:hover > a {
| + | To better understand the inner workings of the <i>Vibrio cholera</i> quorum sensing system we produced a series of computational models to simulate the operation of <i>Vibrio cholerae</i>. Based upon differential equations and solved computationally via the Fourth Order Runge-Kutta method our models provide a comprehensive view of <i>Vibrio cholerae</i> quorum sensing and bi-stable switching behaviour. To find out how we designed our models from the blackboard to the CPU check out our <a href="https://2010.igem.org/Team:St Andrews/project/modelling">modelling </a>. This work is potentially a flexible framework for future quorum sensing modelling. Possible future extensions can be other diffusion models for quorum sensing, other quorum sensing systems, etc. Different models are proposed as an example.</td> |
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| - | background: -moz-linear-gradient(top, #ebebeb, #808080); /* for firefox 3.6+ */
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| - | text-shadow: 0 1px 0 rgba(255, 255, 255, .8);
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| - | /* sub levels link hover */
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| - | * html html>body #nav {
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| - | .f-poweredbyico
| + | <td> |
| - | {
| + | We proposed a new solution for the fine-tuning the translation of proteins designing Ribosome Binding Site by the usage of an RBS calculator. Check out more about this <a href="https://2010.igem.org/Team:St_Andrews/project/ RBS"> RBS</a>. |
| - | position:absolute;
| + | </td> |
| - | }
| + | </tr> |
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| + | </table> |
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| - | color:white;
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| + | |
| - | #toc
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/team/members#Rachael Blackburn">Rachael Blackburn</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/team/members#Lukas Ly">Lukas Ly</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/team/members#Alasdair Morton">Alasdair Morton</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/team/advisors/hooley_chris">Chris Hooley</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/team/advisors/mendivil_olivia">Olivia Mendivil</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/team/advisors/mitchell_john">John Mitchell</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/team/advisors/smith_anne">Anne Smith</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/team/advisors/verleyen_wim">Wim Verleyen</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/project/ objectives"> Objectives</a> </li> | + | |
| - | <li><a href="https://2010.igem.org/Team:St_Andrews/project/laboratory">Laboratory</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/project/modeling">Modeling</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/project/notebook">Notebook</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/project/blog">Blog</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/contact">Contact</a></li>
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| - | <li><a href="https://2010.igem.org/Team:St_Andrews/about">About</a></li>
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| - | Welcome to the University of St Andrew's iGEM wiki!<br>
| + | |
| - | Our multidisciplinary group of scientists will first re-engineer the existing LuxR quorum sensing system to create a bistable switch. This will allow us to infer a signalling molecule concentration required to deactivate the system much lower than the concentration required to activate it.<br>
| + | |
| - | The second part of the project involves introducing the cholera autoinducer synthase gene CqsA to <i>Escherichia coli</i> so that CAI-1 is secreted.<br>
| + | |
| - | Our engineered <i>Escherichia coli</i> will harmlessly colonise the gut, and in large numbers secrete the cholera autoinducer, CAI-1. This will cause an immediate high autoinducer concentration to be detected by incoming <i>Vibrio cholerae</i> cells which then become avirulent and harmlessly pass out of the body.
| + | |
| | </p> | | </p> |
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| |
| | </html> | | </html> |
| | + | * [[Team:St_Andrews/FAQ | Frequently Asked Questions ]] |
| | + | * [https://igem.org/Main_Page iGEM 2010 ] |
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