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ULB/24 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{ULB_Header_2}} '''Quorum addiction module''' *Inventory of all the parts already ready to use. *Midiprep on the RBS strenght 1. *Miniprep on some of the parts needed (AHL degrading enzy...) |
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*Midiprep on the RBS strenght 1. | *Midiprep on the RBS strenght 1. | ||
*Miniprep on some of the parts needed (AHL degrading enzyme, LuxR, Both promoters, LuxI and RBS strenght 0.3). | *Miniprep on some of the parts needed (AHL degrading enzyme, LuxR, Both promoters, LuxI and RBS strenght 0.3). | ||
| + | |||
| + | '''Module hydrogen''' | ||
| + | * After the PCR of verification, we found out that we obtained the ldhA deleted mutant. The other deletion candidate were all negative. | ||
| + | * We continue the purification process for the deletion candidate | ||
| + | * We continue to try to obtain other deletion candidates | ||
| + | * We tested the insertion of tdcE with the RBS. | ||
| + | |||
| + | '''Module homologous recombination''' | ||
| + | * We finally obtained the plasmid TOPO with the cm and kan resistance cassettes ! | ||
| + | * We can now digest the two resistance cassette in order to insert them in the pSB1C3 | ||
| + | |||
| + | |||
| + | '''Module heavy metals detection''' | ||
| + | * We inserted the gene of the carotene behind a constitutive promoter | ||
| + | * We inserted the copper sensitive promoter in front of a RFP | ||
Latest revision as of 02:55, 28 October 2010
Quorum addiction module
- Inventory of all the parts already ready to use.
- Midiprep on the RBS strenght 1.
- Miniprep on some of the parts needed (AHL degrading enzyme, LuxR, Both promoters, LuxI and RBS strenght 0.3).
Module hydrogen
- After the PCR of verification, we found out that we obtained the ldhA deleted mutant. The other deletion candidate were all negative.
- We continue the purification process for the deletion candidate
- We continue to try to obtain other deletion candidates
- We tested the insertion of tdcE with the RBS.
Module homologous recombination
- We finally obtained the plasmid TOPO with the cm and kan resistance cassettes !
- We can now digest the two resistance cassette in order to insert them in the pSB1C3
Module heavy metals detection
- We inserted the gene of the carotene behind a constitutive promoter
- We inserted the copper sensitive promoter in front of a RFP
